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1r80

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{{STRUCTURE_1r80| PDB=1r80 | SCENE= }}
{{STRUCTURE_1r80| PDB=1r80 | SCENE= }}
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'''Glycosyltransferase B in complex with 3-amino-acceptor analog inhibitor and uridine diphosphate'''
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===Glycosyltransferase B in complex with 3-amino-acceptor analog inhibitor and uridine diphosphate===
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==Overview==
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Human ABO(H) blood group glycosyltransferases GTA and GTB catalyze the final monosaccharide addition in the biosynthesis of the human A and B blood group antigens. GTA and GTB utilize a common acceptor, the H antigen disaccharide alpha-l-Fucp-(1--&gt;2)-beta-d-Galp-OR, but different donors, where GTA transfers GalNAc from UDP-GalNAc and GTB transfers Gal from UDP-Gal. GTA and GTB are two of the most homologous enzymes known to transfer different donors and differ in only 4 amino acid residues, but one in particular (Leu/Met-266) has been shown to dominate the selection between donor sugars. The structures of the A and B glycosyltransferases have been determined to high resolution in complex with two inhibitory acceptor analogs alpha-l-Fucp(1--&gt;2)-beta-d-(3-deoxy)-Galp-OR and alpha-l-Fucp-(1--&gt;2)-beta-d-(3-amino)-Galp-OR, in which the 3-hydroxyl moiety of the Gal ring has been replaced by hydrogen or an amino group, respectively. Remarkably, although the 3-deoxy inhibitor occupies the same conformation and position observed for the native H antigen in GTA and GTB, the 3-amino analog is recognized differently by the two enzymes. The 3-amino substitution introduces a novel intramolecular hydrogen bond between O2' on Fuc and N3' on Gal, which alters the minimum-energy conformation of the inhibitor. In the absence of UDP, the 3-amino analog can be accommodated by either GTA or GTB with the l-Fuc residue partially occupying the vacant UDP binding site. However, in the presence of UDP, the analog is forced to abandon the intramolecular hydrogen bond, and the l-Fuc residue is shifted to a less ordered conformation. Further, the residue Leu/Met-266 that was thought important only in distinguishing between donor substrates is observed to interact differently with the 3-amino acceptor analog in GTA and GTB. These observations explain why the 3-deoxy analog acts as a competitive inhibitor of the glycosyltransferase reaction, whereas the 3-amino analog displays complex modes of inhibition.
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{{ABSTRACT_PUBMED_12972418}}
==About this Structure==
==About this Structure==
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[[Category: Signal-anchor]]
[[Category: Signal-anchor]]
[[Category: Transmembrane]]
[[Category: Transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:33:32 2008''

Revision as of 03:33, 29 July 2008

Image:1r80.png

Template:STRUCTURE 1r80

Glycosyltransferase B in complex with 3-amino-acceptor analog inhibitor and uridine diphosphate

Template:ABSTRACT PUBMED 12972418

About this Structure

1R80 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The influence of an intramolecular hydrogen bond in differential recognition of inhibitory acceptor analogs by human ABO(H) blood group A and B glycosyltransferases., Nguyen HP, Seto NO, Cai Y, Leinala EK, Borisova SN, Palcic MM, Evans SV, J Biol Chem. 2003 Dec 5;278(49):49191-5. Epub 2003 Sep 11. PMID:12972418

Page seeded by OCA on Tue Jul 29 06:33:32 2008

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