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2qfj

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{{STRUCTURE_2qfj| PDB=2qfj | SCENE= }}
{{STRUCTURE_2qfj| PDB=2qfj | SCENE= }}
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'''Crystal Structure of First Two RRM Domains of FIR Bound to ssDNA from a Portion of FUSE'''
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===Crystal Structure of First Two RRM Domains of FIR Bound to ssDNA from a Portion of FUSE===
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==Overview==
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c-myc is essential for cell homeostasis and growth but lethal if improperly regulated. Transcription of this oncogene is governed by the counterbalancing forces of two proteins on TFIIH--the FUSE binding protein (FBP) and the FBP-interacting repressor (FIR). FBP and FIR recognize single-stranded DNA upstream of the P1 promoter, known as FUSE, and influence transcription by oppositely regulating TFIIH at the promoter site. Size exclusion chromatography coupled with light scattering reveals that an FIR dimer binds one molecule of single-stranded DNA. The crystal structure confirms that FIR binds FUSE as a dimer, and only the N-terminal RRM domain participates in nucleic acid recognition. Site-directed mutations of conserved residues in the first RRM domain reduce FIR's affinity for FUSE, while analogous mutations in the second RRM domain either destabilize the protein or have no effect on DNA binding. Oppositely oriented DNA on parallel binding sites of the FIR dimer results in spooling of a single strand of bound DNA, and suggests a mechanism for c-myc transcriptional control.
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(as it appears on PubMed at http://www.pubmed.gov), where 18059478 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18059478}}
==About this Structure==
==About this Structure==
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[[Category: Rrm domain]]
[[Category: Rrm domain]]
[[Category: Transcription repressor/dna complex]]
[[Category: Transcription repressor/dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:52:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:33:43 2008''

Revision as of 03:33, 29 July 2008

Image:2qfj.png

Template:STRUCTURE 2qfj

Crystal Structure of First Two RRM Domains of FIR Bound to ssDNA from a Portion of FUSE

Template:ABSTRACT PUBMED 18059478

About this Structure

2QFJ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Dimerization of FIR upon FUSE DNA binding suggests a mechanism of c-myc inhibition., Crichlow GV, Zhou H, Hsiao HH, Frederick KB, Debrosse M, Yang Y, Folta-Stogniew EJ, Chung HJ, Fan C, De la Cruz EM, Levens D, Lolis E, Braddock D, EMBO J. 2008 Jan 9;27(1):277-89. Epub 2007 Dec 6. PMID:18059478

Page seeded by OCA on Tue Jul 29 06:33:43 2008

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