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1rut

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{{STRUCTURE_1rut| PDB=1rut | SCENE= }}
{{STRUCTURE_1rut| PDB=1rut | SCENE= }}
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'''Complex of LMO4 LIM domains 1 and 2 with the ldb1 LID domain'''
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===Complex of LMO4 LIM domains 1 and 2 with the ldb1 LID domain===
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==Overview==
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Nuclear LIM-only (LMO) and LIM-homeodomain (LIM-HD) proteins have important roles in cell fate determination, organ development and oncogenesis. These proteins contain tandemly arrayed LIM domains that bind the LIM interaction domain (LID) of the nuclear adaptor protein LIM domain-binding protein-1 (Ldb1). We have determined a high-resolution X-ray crystal structure of LMO4, a putative breast oncoprotein, in complex with Ldb1-LID, providing the first example of a tandem LIM:Ldb1-LID complex and the first structure of a type-B LIM domain. The complex possesses a highly modular structure with Ldb1-LID binding in an extended manner across both LIM domains of LMO4. The interface contains extensive hydrophobic and electrostatic interactions and multiple backbone-backbone hydrogen bonds. A mutagenic screen of Ldb1-LID, assessed by yeast two-hybrid and competition ELISA analysis, identified key features at the interface and revealed that the interaction is tolerant to mutation. These combined properties provide a mechanism for the binding of Ldb1 to numerous LMO and LIM-HD proteins. Furthermore, the modular extended interface may form a general mode of binding to tandem LIM domains.
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(as it appears on PubMed at http://www.pubmed.gov), where 15343268 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15343268}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Tandem LIM domains provide synergistic binding in the LMO4:Ldb1 complex., Deane JE, Ryan DP, Sunde M, Maher MJ, Guss JM, Visvader JE, Matthews JM, EMBO J. 2004 Sep 15;23(18):3589-98. Epub 2004 Sep 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15343268 15343268]
Tandem LIM domains provide synergistic binding in the LMO4:Ldb1 complex., Deane JE, Ryan DP, Sunde M, Maher MJ, Guss JM, Visvader JE, Matthews JM, EMBO J. 2004 Sep 15;23(18):3589-98. Epub 2004 Sep 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15343268 15343268]
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Crystallization of FLINC4, an intramolecular LMO4-ldb1 complex., Deane JE, Maher MJ, Langley DB, Graham SC, Visvader JE, Guss JM, Matthews JM, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1484-6. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876360 12876360]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Fusion protein]]
[[Category: Fusion protein]]
[[Category: Lim domain]]
[[Category: Lim domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:56:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:37:58 2008''

Revision as of 03:37, 29 July 2008

Image:1rut.png

Template:STRUCTURE 1rut

Complex of LMO4 LIM domains 1 and 2 with the ldb1 LID domain

Template:ABSTRACT PUBMED 15343268

About this Structure

1RUT is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Tandem LIM domains provide synergistic binding in the LMO4:Ldb1 complex., Deane JE, Ryan DP, Sunde M, Maher MJ, Guss JM, Visvader JE, Matthews JM, EMBO J. 2004 Sep 15;23(18):3589-98. Epub 2004 Sep 2. PMID:15343268

Crystallization of FLINC4, an intramolecular LMO4-ldb1 complex., Deane JE, Maher MJ, Langley DB, Graham SC, Visvader JE, Guss JM, Matthews JM, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1484-6. Epub 2003, Jul 23. PMID:12876360

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