2flh

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{{STRUCTURE_2flh| PDB=2flh | SCENE= }}
{{STRUCTURE_2flh| PDB=2flh | SCENE= }}
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'''Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin'''
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===Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin===
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==Overview==
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The cytosolic fraction of Vigna radiata contains a 17-kD protein that binds plant hormones from the cytokinin group, such as zeatin. Using recombinant protein and isothermal titration calorimetry as well as fluorescence measurements coupled with ligand displacement, we have reexamined the K(d) values and show them to range from approximately 10(-6) M (for 4PU30) to 10(-4) M (for zeatin) for 1:1 stoichiometry complexes. In addition, we have crystallized this cytokinin-specific binding protein (Vr CSBP) in complex with zeatin and refined the structure to 1.2 A resolution. Structurally, Vr CSBP is similar to plant pathogenesis-related class 10 (PR-10) proteins, despite low sequence identity (&lt;20%). This unusual fold conservation reinforces the notion that classic PR-10 proteins have evolved to bind small-molecule ligands. The fold consists of an antiparallel beta-sheet wrapped around a C-terminal alpha-helix, with two short alpha-helices closing a cavity formed within the protein core. In each of the four independent CSBP molecules, there is a zeatin ligand located deep in the cavity with conserved conformation and protein-ligand interactions. In three cases, an additional zeatin molecule is found in variable orientation but with excellent definition in electron density, which plugs the entrance to the binding pocket, sealing the inner molecule from contact with bulk solvent.
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The line below this paragraph, {{ABSTRACT_PUBMED_16998071}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16998071 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16998071}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin., Pasternak O, Bujacz GD, Fujimoto Y, Hashimoto Y, Jelen F, Otlewski J, Sikorski MM, Jaskolski M, Plant Cell. 2006 Oct;18(10):2622-34. Epub 2006 Sep 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16998071 16998071]
Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin., Pasternak O, Bujacz GD, Fujimoto Y, Hashimoto Y, Jelen F, Otlewski J, Sikorski MM, Jaskolski M, Plant Cell. 2006 Oct;18(10):2622-34. Epub 2006 Sep 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16998071 16998071]
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Crystallization and preliminary crystallographic studies of mung bean cytokinin-specific binding protein., Bujacz GD, Pasternak O, Fujimoto Y, Hashimoto Y, Sikorski MM, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):522-5. Epub 2003, Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12595714 12595714]
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Purification and cDNA cloning of cytokinin-specific binding protein from mung bean (Vigna radiata)., Fujimoto Y, Nagata R, Fukasawa H, Yano K, Azuma M, Iida A, Sugimoto S, Shudo K, Hashimoto Y, Eur J Biochem. 1998 Dec 1;258(2):794-802. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9874249 9874249]
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Crystal structures of two homologous pathogenesis-related proteins from yellow lupine., Biesiadka J, Bujacz G, Sikorski MM, Jaskolski M, J Mol Biol. 2002 Jun 21;319(5):1223-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12079359 12079359]
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Structure of a yellow lupin pathogenesis-related PR-10 protein belonging to a novel subclass., Pasternak O, Biesiadka J, Dolot R, Handschuh L, Bujacz G, Sikorski MM, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):99-107. Epub 2004, Dec 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15608381 15608381]
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X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy., Gajhede M, Osmark P, Poulsen FM, Ipsen H, Larsen JN, Joost van Neerven RJ, Schou C, Lowenstein H, Spangfort MD, Nat Struct Biol. 1996 Dec;3(12):1040-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8946858 8946858]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Vigna radiata]]
[[Category: Vigna radiata]]
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[[Category: Plant protein]]
[[Category: Plant protein]]
[[Category: Zeatin]]
[[Category: Zeatin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:22:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:42:54 2008''

Revision as of 03:42, 29 July 2008

Image:2flh.png

Template:STRUCTURE 2flh

Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin

Template:ABSTRACT PUBMED 16998071

About this Structure

2FLH is a Single protein structure of sequence from Vigna radiata. Full crystallographic information is available from OCA.

Reference

Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin., Pasternak O, Bujacz GD, Fujimoto Y, Hashimoto Y, Jelen F, Otlewski J, Sikorski MM, Jaskolski M, Plant Cell. 2006 Oct;18(10):2622-34. Epub 2006 Sep 22. PMID:16998071

Crystallization and preliminary crystallographic studies of mung bean cytokinin-specific binding protein., Bujacz GD, Pasternak O, Fujimoto Y, Hashimoto Y, Sikorski MM, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):522-5. Epub 2003, Feb 21. PMID:12595714

Purification and cDNA cloning of cytokinin-specific binding protein from mung bean (Vigna radiata)., Fujimoto Y, Nagata R, Fukasawa H, Yano K, Azuma M, Iida A, Sugimoto S, Shudo K, Hashimoto Y, Eur J Biochem. 1998 Dec 1;258(2):794-802. PMID:9874249

Crystal structures of two homologous pathogenesis-related proteins from yellow lupine., Biesiadka J, Bujacz G, Sikorski MM, Jaskolski M, J Mol Biol. 2002 Jun 21;319(5):1223-34. PMID:12079359

Structure of a yellow lupin pathogenesis-related PR-10 protein belonging to a novel subclass., Pasternak O, Biesiadka J, Dolot R, Handschuh L, Bujacz G, Sikorski MM, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):99-107. Epub 2004, Dec 17. PMID:15608381

X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy., Gajhede M, Osmark P, Poulsen FM, Ipsen H, Larsen JN, Joost van Neerven RJ, Schou C, Lowenstein H, Spangfort MD, Nat Struct Biol. 1996 Dec;3(12):1040-5. PMID:8946858

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