1pdx
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(New page: 200px<br /><applet load="1pdx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pdx" /> '''PUTIDAREDOXIN'''<br /> ==Overview== A refin...)
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Revision as of 21:36, 20 November 2007
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PUTIDAREDOXIN
Overview
A refined model for the solution structure of oxidized putidaredoxin, (Pdxo), a Cys4Fe2S2 ferredoxin, has been determined. A previous structure, (Pochapsky et al. (1994) Biochemistry 33, 6424-6432; PDB entry ) was, calculated using the results of homonuclear two-dimensional NMR, experiments. New data has made it possible to calculate a refinement of, the original Pdxo solution structure. First, essentially complete, assignments for diamagnetic 15N and 13C resonances of Pdxo have been made, using multidimensional NMR methods, and 15N- and 13C-resolved NOESY, experiments have permitted the identification of many new NOE restraints, for structural calculations. Stereospecific assignments for leucine and, valine CH3 resonances were made using biosynthetically directed fractional, 13C labeling, improving the precision of NOE restraints involving these, residues. Backbone dihedral angle restraints have been obtained using a, combination of two-dimensional J-modulated 15N,1H HSQC and 3D (HN)CO(CO)NH, experiments. Second, the solution structure of a diamagnetic form of Pdx, that of the C85S variant of gallium putidaredoxin, in which a nonligand, Cys is replaced by Ser, has been determined (Pochapsky et al. (1998) J., Biomol. NMR 12, 407-415), providing information concerning structural, features not observable in the native ferredoxin due to paramagnetism., Third, a crystal structure of a closely related ferredoxin, bovine, adrenodoxin, has been published (Muller et al. (1998) Structure 6, 269-280). This structure has been used to model the metal binding site, structure in Pdx. A family of fourteen structures is presented that, exhibits an rmsd of 0.51 A for backbone heavy atoms and 0.83 A for all, heavy atoms. Exclusion of the modeled metal binding loop region reduces, overall the rmsd to 0.30 A for backbone atoms and 0.71 A for all heavy, atoms.
About this Structure
1PDX is a Single protein structure of sequence from Pseudomonas putida with FES as ligand. Full crystallographic information is available from OCA.
Reference
A refined model for the solution structure of oxidized putidaredoxin., Pochapsky TC, Jain NU, Kuti M, Lyons TA, Heymont J, Biochemistry. 1999 Apr 13;38(15):4681-90. PMID:10200155
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