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2fc1

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[[Image:2fc1.gif|left|200px]]
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{{STRUCTURE_2fc1| PDB=2fc1 | SCENE= }}
{{STRUCTURE_2fc1| PDB=2fc1 | SCENE= }}
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'''Heme NO Complex in NOS'''
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===Heme NO Complex in NOS===
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==Overview==
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The crystal structures of nitrosyl-heme complexes of a prokaryotic nitric oxide synthase (NOS) from Bacillus subtilis (bsNOS) reveal changes in active-site hydrogen bonding in the presence of the intermediate N(omega)-hydroxy-l-arginine (NOHA) compared to the substrate l-arginine (l-Arg). Correlating with a Val-to-Ile residue substitution in the bsNOS heme pocket, the Fe(II)-NO complex with both l-Arg and NOHA is more bent than the Fe(II)-NO, l-Arg complex of mammalian eNOS [Li, H., Raman, C. S., Martasek, P., Masters, B. S. S., and Poulos, T. L. (2001) Biochemistry 40, 5399-5406]. Structures of the Fe(III)-NO complex with NOHA show a nearly linear nitrosyl group, and in one subunit, partial nitrosation of bound NOHA. In the Fe(II)-NO complexes, the protonated NOHA N(omega) atom forms a short hydrogen bond with the heme-coordinated NO nitrogen, but active-site water molecules are out of hydrogen bonding range with the distal NO oxygen. In contrast, the l-Arg guanidinium interacts more weakly and equally with both NO atoms, and an active-site water molecule hydrogen bonds to the distal NO oxygen. This difference in hydrogen bonding to the nitrosyl group by the two substrates indicates that interactions provided by NOHA may preferentially stabilize an electrophilic peroxo-heme intermediate in the second step of NOS catalysis.
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(as it appears on PubMed at http://www.pubmed.gov), where 16489746 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16489746}}
==About this Structure==
==About this Structure==
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[[Category: Heme-no complex]]
[[Category: Heme-no complex]]
[[Category: Nitric oxide synthase]]
[[Category: Nitric oxide synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:42:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:52:18 2008''

Revision as of 03:52, 29 July 2008

Image:2fc1.png

Template:STRUCTURE 2fc1

Heme NO Complex in NOS

Template:ABSTRACT PUBMED 16489746

About this Structure

2FC1 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Nitrosyl-heme structures of Bacillus subtilis nitric oxide synthase have implications for understanding substrate oxidation., Pant K, Crane BR, Biochemistry. 2006 Feb 28;45(8):2537-44. PMID:16489746

Page seeded by OCA on Tue Jul 29 06:52:18 2008

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