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2ftk

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[[Image:2ftk.gif|left|200px]]
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{{Seed}}
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[[Image:2ftk.png|left|200px]]
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{{STRUCTURE_2ftk| PDB=2ftk | SCENE= }}
{{STRUCTURE_2ftk| PDB=2ftk | SCENE= }}
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'''berylloflouride Spo0F complex with Spo0B'''
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===berylloflouride Spo0F complex with Spo0B===
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==Overview==
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A number of regulatory circuits in biological systems function through the exchange of phosphoryl groups from one protein to another. Spo0F and Spo0B are components of a phosphorelay that control sporulation in the bacterium Bacillus subtilis through the exchange of a phosphoryl group. Using beryllofluoride as a mimic for phosphorylation, we trapped the interaction of the phosphorylated Spo0F with Spo0B in the crystal lattice. The transition state of phosphoryl transfer continues to be a highly debated issue, as to whether it is associative or dissociative in nature. The geometry of Spo0F binding to Spo0B favors an associative mechanism for phosphoryl transfer. In order to visualize the autophosphorylation of the histidine kinase, KinA, and the subsequent phosphoryl transfer to Spo0F, we generated in silico models representing these reaction steps.
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(as it appears on PubMed at http://www.pubmed.gov), where 16788205 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16788205}}
==About this Structure==
==About this Structure==
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[[Category: Spo0f]]
[[Category: Spo0f]]
[[Category: Sporulation]]
[[Category: Sporulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:17:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:53:11 2008''

Revision as of 03:53, 29 July 2008

Image:2ftk.png

Template:STRUCTURE 2ftk

berylloflouride Spo0F complex with Spo0B

Template:ABSTRACT PUBMED 16788205

About this Structure

2FTK is a Protein complex structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

The crystal structure of beryllofluoride Spo0F in complex with the phosphotransferase Spo0B represents a phosphotransfer pretransition state., Varughese KI, Tsigelny I, Zhao H, J Bacteriol. 2006 Jul;188(13):4970-7. PMID:16788205

Page seeded by OCA on Tue Jul 29 06:53:11 2008

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