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1qsr

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{{STRUCTURE_1qsr| PDB=1qsr | SCENE= }}
{{STRUCTURE_1qsr| PDB=1qsr | SCENE= }}
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'''CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND ACETYL-COENZYME A'''
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===CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND ACETYL-COENZYME A===
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==Overview==
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Gene activation is a highly regulated process that requires the coordinated action of proteins to relieve chromatin repression and to promote transcriptional activation. Nuclear histone acetyltransferase (HAT) enzymes provide a mechanistic link between chromatin destabilization and gene activation by acetylating the epsilon-amino group of specific lysine residues within the aminoterminal tails of core histones to facilitate access to DNA by transcriptional activators. Here we report the high-resolution crystal structure of the HAT domain of Tetrahymena GCN5 (tGCN5) bound with both its physiologically relevant ligands, coenzyme A (CoA) and a histone H3 peptide, and the structures of nascent tGCN5 and a tGCN5/acetyl-CoA complex. Our structural data reveal histone-binding specificity for a random-coil structure containing a G-K-X-P recognition sequence, and show that CoA is essential for reorienting the enzyme for histone binding. Catalysis appears to involve water-mediated proton extraction from the substrate lysine by a glutamic acid general base and a backbone amide that stabilizes the transition-state reaction intermediate. Comparison with related N-acetyltransferases indicates a conserved structural framework for CoA binding and catalysis, and structural variability in regions associated with substrate-specific binding.
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(as it appears on PubMed at http://www.pubmed.gov), where 10485713 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10485713}}
==About this Structure==
==About this Structure==
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[[Category: Gcn5-related n-acetyltransferase]]
[[Category: Gcn5-related n-acetyltransferase]]
[[Category: Histone acetyltransferase]]
[[Category: Histone acetyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:39:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:55:19 2008''

Revision as of 03:55, 29 July 2008

Image:1qsr.png

Template:STRUCTURE 1qsr

CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND ACETYL-COENZYME A

Template:ABSTRACT PUBMED 10485713

About this Structure

1QSR is a Single protein structure of sequence from Tetrahymena thermophila. Full crystallographic information is available from OCA.

Reference

Structure of Tetrahymena GCN5 bound to coenzyme A and a histone H3 peptide., Rojas JR, Trievel RC, Zhou J, Mo Y, Li X, Berger SL, Allis CD, Marmorstein R, Nature. 1999 Sep 2;401(6748):93-8. PMID:10485713

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