1ua3

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[[Image:1ua3.jpg|left|200px]]
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{{Seed}}
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[[Image:1ua3.png|left|200px]]
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{{STRUCTURE_1ua3| PDB=1ua3 | SCENE= }}
{{STRUCTURE_1ua3| PDB=1ua3 | SCENE= }}
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'''Crystal structure of the pig pancreatic a-amylase complexed with malto-oligosaccharides'''
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===Crystal structure of the pig pancreatic a-amylase complexed with malto-oligosaccharides===
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==Overview==
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The structural X-ray map of a pig pancreatic alpha-amylase crystal soaked (and flash-frozen) with a maltopentaose substrate showed a pattern of electron density corresponding to the binding of oligosaccharides at the active site and at three surface binding sites. The electron density region observed at the active site, filling subsites-3 through-1, was interpreted in terms of the process of enzyme-catalyzed hydrolysis undergone by maltopentaose. Because the expected conformational changes in the "flexible loop" that constitutes the surface edge of the active site were not observed, the movement of the loop may depend on aglycone site being filled. The crystal structure was refined at 2.01 A resolution to an R factor of 17.0% ( R(free) factor of 19.8%). The final model consists of 3910 protein atoms, one calcium ion, two chloride ions, 103 oligosaccharide atoms, 761 atoms of water molecules, and 23 ethylene glycol atoms.
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The line below this paragraph, {{ABSTRACT_PUBMED_12962327}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12962327 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12962327}}
==About this Structure==
==About this Structure==
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[[Category: Qian, M.]]
[[Category: Qian, M.]]
[[Category: Beta-alpha-barrel]]
[[Category: Beta-alpha-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:57:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:55:53 2008''

Revision as of 03:55, 29 July 2008

Image:1ua3.png

Template:STRUCTURE 1ua3

Crystal structure of the pig pancreatic a-amylase complexed with malto-oligosaccharides

Template:ABSTRACT PUBMED 12962327

About this Structure

1UA3 is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Crystal structure of the pig pancreatic alpha-amylase complexed with malto-oligosaccharides., Payan F, Qian M, J Protein Chem. 2003 Apr;22(3):275-84. PMID:12962327

Page seeded by OCA on Tue Jul 29 06:55:53 2008

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