This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2had

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2had.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2had.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2had| PDB=2had | SCENE= }}
{{STRUCTURE_2had| PDB=2had | SCENE= }}
-
'''CRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE: AN ENZYME TO DETOXIFY HALOGENATED ALKANES'''
+
===CRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE: AN ENZYME TO DETOXIFY HALOGENATED ALKANES===
-
==Overview==
+
<!--
-
Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 converts 1-haloalkanes to the corresponding alcohols and halide ions with water as the sole cosubstrate and without any need for oxygen or cofactors. The three-dimensional structure has been determined by multiple isomorphous replacement techniques using three heavy atom derivatives. The structure has been refined at 2.4 A resolution to an R-factor of 17.9%. The monomeric enzyme is a spherical molecule and is composed to two domains: domain I has an alpha/beta type structure with a central eight-stranded mainly parallel beta-sheet. Domain II lies like a cap on top of domain I and consists of alpha-helices connected by loops. Except for the cap domain the structure resembles that of the dienelactone hydrolase in spite of any significant sequence homology. The putative active site is completely buried in an internal hydrophobic cavity which is located between the two domains. From the analysis of the structure it is suggested that Asp124 is the nucleophilic residue essential for the catalysis. It interacts with His289 which is hydrogen-bonded to Asp260.
+
The line below this paragraph, {{ABSTRACT_PUBMED_2026135}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 2026135 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_2026135}}
==About this Structure==
==About this Structure==
Line 26: Line 30:
[[Category: Verschueren, K H.G.]]
[[Category: Verschueren, K H.G.]]
[[Category: Dehalogenase]]
[[Category: Dehalogenase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:03:07 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:56:01 2008''

Revision as of 03:56, 29 July 2008

Image:2had.png

Template:STRUCTURE 2had

CRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE: AN ENZYME TO DETOXIFY HALOGENATED ALKANES

Template:ABSTRACT PUBMED 2026135

About this Structure

2HAD is a Single protein structure of sequence from Xanthobacter autotrophicus. Full crystallographic information is available from OCA.

Reference

Crystal structure of haloalkane dehalogenase: an enzyme to detoxify halogenated alkanes., Franken SM, Rozeboom HJ, Kalk KH, Dijkstra BW, EMBO J. 1991 Jun;10(6):1297-302. PMID:2026135

Page seeded by OCA on Tue Jul 29 06:56:01 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2had"
Personal tools