2jsx

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[[Image:2jsx.jpg|left|200px]]
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{{Seed}}
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[[Image:2jsx.png|left|200px]]
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{{STRUCTURE_2jsx| PDB=2jsx | SCENE= }}
{{STRUCTURE_2jsx| PDB=2jsx | SCENE= }}
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'''Solution structure of the E. coli Tat proofreading chaperone protein NapD'''
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===Solution structure of the E. coli Tat proofreading chaperone protein NapD===
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==Overview==
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The twin-arginine transport (Tat) system is dedicated to the translocation of folded proteins across the bacterial cytoplasmic membrane. Proteins are targeted to the Tat system by signal peptides containing a twin-arginine motif. In Escherichia coli, many Tat substrates bind redox-active cofactors in the cytoplasm before transport. Coordination of cofactor insertion with protein export involves a "Tat proofreading" process in which chaperones bind twin-arginine signal peptides, thus preventing premature export. The initial Tat signal-binding proteins described belonged to the TorD family, which are required for assembly of N- and S-oxide reductases. Here, we report that E. coli NapD is a Tat signal peptide-binding chaperone involved in biosynthesis of the Tat-dependent nitrate reductase NapA. NapD binds tightly and specifically to the NapA twin-arginine signal peptide and suppresses signal peptide translocation activity such that transport via the Tat pathway is retarded. High-resolution, heteronuclear, multidimensional NMR spectroscopy reveals the 3D solution structure of NapD. The chaperone adopts a ferredoxin-type fold, which is completely distinct from the TorD family. Thus, NapD represents a new family of twin-arginine signal-peptide-binding proteins.
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The line below this paragraph, {{ABSTRACT_PUBMED_17901208}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17901208 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17901208}}
==About this Structure==
==About this Structure==
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2JSX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JSX OCA].
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2JSX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JSX OCA].
==Reference==
==Reference==
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[[Category: Protein]]
[[Category: Protein]]
[[Category: Tat]]
[[Category: Tat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:15:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:58:12 2008''

Revision as of 03:58, 29 July 2008

Image:2jsx.png

Template:STRUCTURE 2jsx

Solution structure of the E. coli Tat proofreading chaperone protein NapD

Template:ABSTRACT PUBMED 17901208

About this Structure

2JSX is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Structural diversity in twin-arginine signal peptide-binding proteins., Maillard J, Spronk CA, Buchanan G, Lyall V, Richardson DJ, Palmer T, Vuister GW, Sargent F, Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15641-6. Epub 2007 Sep 27. PMID:17901208

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