2afn

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{{STRUCTURE_2afn| PDB=2afn | SCENE= }}
{{STRUCTURE_2afn| PDB=2afn | SCENE= }}
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'''STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC'''
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===STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC===
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==Overview==
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The structures at 2.0 and 2.25 A resolution of native and recombinant nitrite reductase from Alcaligenes faecalis show that they are identical to each other and very similar to nitrite reductase from Achromobacter cycloclastes. The crystallographic structure of a mutant, M150E, which unlike the wild-type protein cannot be reduced by pseudoazurin, shows that the glutamate replacement for methionine binds to a metal at the type I Cu site via only one oxygen. Anomalous scattering data collected at wavelengths of 1.040 and 1.377 A reveal that the metal at the type I site is a Zn. No significant differences from the native structure other than local perturbations at the type I site are seen. A local pseudo 2-fold axis relates the two domains of different monomers which form the active site. The two residues, Asp98 and His255, believed to be involved in catalysis are related by this 2-fold. An unusual (+)-(+) charge interaction between Lys269, Glu279, and His100 helps to orient the active site Cu ligand, His100. A number of negatively charged surface residues create an electrostatic field whose shape suggests that it may serve to direct incoming negatively charged nitrite as well as to dock the electron donor partner, pseudoazurin.
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(as it appears on PubMed at http://www.pubmed.gov), where 7547950 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7547950}}
==About this Structure==
==About this Structure==
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[[Category: Nitrite assimilation]]
[[Category: Nitrite assimilation]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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Revision as of 03:59, 29 July 2008

Image:2afn.png

Template:STRUCTURE 2afn

STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC

Template:ABSTRACT PUBMED 7547950

About this Structure

2AFN is a Single protein structure of sequence from Alcaligenes faecalis. This structure supersedes the now removed PDB entry 1afn. Full crystallographic information is available from OCA.

Reference

Structure of Alcaligenes faecalis nitrite reductase and a copper site mutant, M150E, that contains zinc., Murphy ME, Turley S, Kukimoto M, Nishiyama M, Horinouchi S, Sasaki H, Tanokura M, Adman ET, Biochemistry. 1995 Sep 26;34(38):12107-17. PMID:7547950

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