1u0b

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[[Image:1u0b.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1u0b| PDB=1u0b | SCENE= }}
{{STRUCTURE_1u0b| PDB=1u0b | SCENE= }}
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'''Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys'''
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===Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys===
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==Overview==
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The crystal structure of Escherichia coli cysteinyl-tRNA synthetase (CysRS) bound to tRNA(Cys) at a resolution of 2.3 A reveals base-specific and shape-selective interactions across an extensive protein-RNA recognition interface. The complex contains a mixed alpha/beta C-terminal domain, which is disordered in the unliganded enzyme. This domain makes specific hydrogen bonding interactions with all three bases of the GCA anticodon. The tRNA anticodon stem is bent sharply toward the enzyme as compared with its conformation when bound to elongation factor Tu, providing an essential basis for shape-selective recognition. The CysRS structure also reveals interactions of conserved enzyme groups with the sugar-phosphate backbone in the D loop, adjacent to an unusual G15.G48 tertiary base pair previously implicated in tRNA aminoacylation. A combined mutational analysis of enzyme and tRNA groups at G15.G48 supports the notion that contacts between CysRS and the sugar-phosphate backbone contribute to recognition by indirect readout.
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The line below this paragraph, {{ABSTRACT_PUBMED_15489861}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15489861 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15489861}}
==About this Structure==
==About this Structure==
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[[Category: Zhang, C M.]]
[[Category: Zhang, C M.]]
[[Category: Protein-rna complex]]
[[Category: Protein-rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:35:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:02:11 2008''

Revision as of 04:02, 29 July 2008

Image:1u0b.png

Template:STRUCTURE 1u0b

Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys

Template:ABSTRACT PUBMED 15489861

About this Structure

1U0B is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Shape-selective RNA recognition by cysteinyl-tRNA synthetase., Hauenstein S, Zhang CM, Hou YM, Perona JJ, Nat Struct Mol Biol. 2004 Nov;11(11):1134-41. Epub 2004 Oct 17. PMID:15489861

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