2h1v

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2h1v.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2h1v.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2h1v| PDB=2h1v | SCENE= }}
{{STRUCTURE_2h1v| PDB=2h1v | SCENE= }}
-
'''Crystal structure of the Lys87Ala mutant variant of Bacillus subtilis ferrochelatase'''
+
===Crystal structure of the Lys87Ala mutant variant of Bacillus subtilis ferrochelatase===
-
==Overview==
+
<!--
-
Ferrochelatase catalyzes the terminal step in the heme biosynthetic pathway, i.e., the incorporation of Fe(II) into protoporphyrin IX. Various biochemical and biophysical methods have been used to probe the enzyme for metal binding residues and the location of the active site. However, the location of the metal binding site and the path of the metal into the porphyrin are still disputed. Using site-directed mutagenesis on Bacillus subtilis ferrochelatase we demonstrate that exchange of the conserved residues His183 and Glu264 affects the metal affinity of the enzyme. We also present the first X-ray crystal structure of ferrochelatase with iron. Only a single iron was found in the active site, coordinated in a square pyramidal fashion by two amino acid residues, His183 and Glu264, and three water molecules. This iron was not present in the structure of a His183Ala modified ferrochelatase. The results strongly suggest that the insertion of a metal ion into protoporphyrin IX by ferrochelatase occurs from a metal binding site represented by His183 and Glu264.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17198378}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17198378 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17198378}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Pi-helix]]
[[Category: Pi-helix]]
[[Category: Rossman fold]]
[[Category: Rossman fold]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:46:28 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:08:34 2008''

Revision as of 04:08, 29 July 2008

Image:2h1v.png

Template:STRUCTURE 2h1v

Crystal structure of the Lys87Ala mutant variant of Bacillus subtilis ferrochelatase

Template:ABSTRACT PUBMED 17198378

About this Structure

2H1V is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Amino acid residues His183 and Glu264 in Bacillus subtilis ferrochelatase direct and facilitate the insertion of metal ion into protoporphyrin IX., Hansson MD, Karlberg T, Rahardja MA, Al-Karadaghi S, Hansson M, Biochemistry. 2007 Jan 9;46(1):87-94. PMID:17198378

Page seeded by OCA on Tue Jul 29 07:08:34 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2h1v"
Personal tools