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1sg4

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{{STRUCTURE_1sg4| PDB=1sg4 | SCENE= }}
{{STRUCTURE_1sg4| PDB=1sg4 | SCENE= }}
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'''Crystal structure of human mitochondrial delta3-delta2-enoyl-CoA isomerase'''
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===Crystal structure of human mitochondrial delta3-delta2-enoyl-CoA isomerase===
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==Overview==
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The crystal structure of Delta3-Delta2-enoyl-CoA isomerase from human mitochondria (hmEci), complexed with the substrate analogue octanoyl-CoA, has been refined at 1.3 A resolution. This enzyme takes part in the beta-oxidation of unsaturated fatty acids by converting both cis-3 and trans-3-enoyl-CoA esters (with variable length of the acyl group) to trans-2-enoyl-CoA. hmEci belongs to the hydratase/isomerase (crotonase) superfamily. Most of the enzymes belonging to this superfamily are hexamers, but hmEci is shown to be a trimer. The mode of binding of the ligand, octanoyl-CoA, shows that the omega-end of the acyl group binds in a hydrophobic tunnel formed by residues of the loop preceding helix H4 as well as by side-chains of the kinked helix H9. From the structure of the complex it can be seen that Glu136 is the only catalytic residue. The importance of Glu136 for catalysis is confirmed by mutagenesis studies. A cavity analysis shows the presence of two large, adjacent empty hydrophobic cavities near the active site, which are shaped by side-chains of helices H1, H2, H3 and H4. The structure comparison of hmEci with structures of other superfamily members, in particular of rat mitochondrial hydratase (crotonase) and yeast peroxisomal enoyl-CoA isomerase, highlights the variable mode of binding of the fatty acid moiety in this superfamily.
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(as it appears on PubMed at http://www.pubmed.gov), where 15351645 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15351645}}
==About this Structure==
==About this Structure==
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[[Category: Wierenga, R K.]]
[[Category: Wierenga, R K.]]
[[Category: Crotonase fold]]
[[Category: Crotonase fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:39:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:11:03 2008''

Revision as of 04:11, 29 July 2008

Image:1sg4.png

Template:STRUCTURE 1sg4

Crystal structure of human mitochondrial delta3-delta2-enoyl-CoA isomerase

Template:ABSTRACT PUBMED 15351645

About this Structure

1SG4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The 1.3 A crystal structure of human mitochondrial Delta3-Delta2-enoyl-CoA isomerase shows a novel mode of binding for the fatty acyl group., Partanen ST, Novikov DK, Popov AN, Mursula AM, Hiltunen JK, Wierenga RK, J Mol Biol. 2004 Sep 24;342(4):1197-208. PMID:15351645

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