1leo

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{{STRUCTURE_1leo| PDB=1leo | SCENE= }}
{{STRUCTURE_1leo| PDB=1leo | SCENE= }}
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'''P100S NUCLEOSIDE DIPHOSPHATE KINASE'''
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===P100S NUCLEOSIDE DIPHOSPHATE KINASE===
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==Overview==
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NDP kinase from Dictyostelium was mutated by site-directed mutagenesis at positions indicated by structural data to be involved in the trimer interface. The mutants were substitutions at residue Pro-100 (P100S and P100G) and deletions of 1-5 residues at the C terminus. Single mutants yielded proteins that kept both activity and hexameric structure. However, they were severely affected in their stability toward temperature and urea denaturation. When the P100S mutation was combined with any of the C-terminal deletions, the enzyme lost most of its activity and dissociated into dimers. Crystallographic analysis of the P100S protein was performed at 2.6 A resolution. The x-ray structure showed no direct alteration of intersubunits contacts at residue 100, but an induced disruption of the interaction between Asp-115 and the C terminus of another subunit. The substitution of proline 100 to serine corresponds to the Killer-of-prune mutation in Drosophila. Consequences of the mutation are discussed in view of the structural and biochemical properties observed in the mutant Dictyostelium protein.
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{{ABSTRACT_PUBMED_8702707}}
==About this Structure==
==About this Structure==
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[[Category: Kinase]]
[[Category: Kinase]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:20:29 2008''

Revision as of 04:20, 29 July 2008

Image:1leo.png

Template:STRUCTURE 1leo

P100S NUCLEOSIDE DIPHOSPHATE KINASE

Template:ABSTRACT PUBMED 8702707

About this Structure

1LEO is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.

Reference

Nucleoside diphosphate kinase. Investigation of the intersubunit contacts by site-directed mutagenesis and crystallography., Karlsson A, Mesnildrey S, Xu Y, Morera S, Janin J, Veron M, J Biol Chem. 1996 Aug 16;271(33):19928-34. PMID:8702707

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