1zm3

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[[Image:1zm3.gif|left|200px]]
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{{Seed}}
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[[Image:1zm3.png|left|200px]]
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{{STRUCTURE_1zm3| PDB=1zm3 | SCENE= }}
{{STRUCTURE_1zm3| PDB=1zm3 | SCENE= }}
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'''Structure of the apo eEF2-ETA complex'''
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===Structure of the apo eEF2-ETA complex===
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==Overview==
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The bacteria causing diphtheria, whooping cough, cholera and other diseases secrete mono-ADP-ribosylating toxins that modify intracellular proteins. Here, we describe four structures of a catalytically active complex between a fragment of Pseudomonas aeruginosa exotoxin A (ETA) and its protein substrate, translation elongation factor 2 (eEF2). The target residue in eEF2, diphthamide (a modified histidine), spans across a cleft and faces the two phosphates and a ribose of the non-hydrolysable NAD+ analogue, betaTAD. This suggests that the diphthamide is involved in triggering NAD+ cleavage and interacting with the proposed oxacarbenium intermediate during the nucleophilic substitution reaction, explaining the requirement of diphthamide for ADP ribosylation. Diphtheria toxin may recognize eEF2 in a manner similar to ETA. Notably, the toxin-bound betaTAD phosphates mimic the phosphate backbone of two nucleotides in a conformational switch of 18S rRNA, thereby achieving universal recognition of eEF2 by ETA.
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The line below this paragraph, {{ABSTRACT_PUBMED_16107839}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16107839 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16107839}}
==About this Structure==
==About this Structure==
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[[Category: Elongation factor]]
[[Category: Elongation factor]]
[[Category: Toxin]]
[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:47:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:22:26 2008''

Revision as of 04:22, 29 July 2008

Image:1zm3.png

Template:STRUCTURE 1zm3

Structure of the apo eEF2-ETA complex

Template:ABSTRACT PUBMED 16107839

About this Structure

1ZM3 is a Protein complex structure of sequences from Pseudomonas aeruginosa and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Exotoxin A-eEF2 complex structure indicates ADP ribosylation by ribosome mimicry., Jorgensen R, Merrill AR, Yates SP, Marquez VE, Schwan AL, Boesen T, Andersen GR, Nature. 2005 Aug 18;436(7053):979-84. PMID:16107839

Page seeded by OCA on Tue Jul 29 07:22:26 2008

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