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1xft

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{{STRUCTURE_1xft| PDB=1xft | SCENE= }}
{{STRUCTURE_1xft| PDB=1xft | SCENE= }}
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'''Synchrotron X-ray Powder Diffraction Study of Hexagonal Turkey Egg-white Lysozyme'''
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===Synchrotron X-ray Powder Diffraction Study of Hexagonal Turkey Egg-white Lysozyme===
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==Overview==
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The structure of turkey egg-white lysozyme (TEWL) has been refined from high-resolution X-ray powder diffraction data. The sample was rapidly obtained as a polycrystalline precipitate at high protein concentration using 0.5 M NaCl solvent pH 6 and was deposited in the PDB with code 1xft. The diffraction data were collected at room temperature. Molecular replacement was shown to give a suitable starting point for refinement, illustrating that powder data can be sufficient for this approach. Crystallographic models were then refined by combined Rietveld and stereochemical restraint analysis of the powder data (d(min) = 3.35 A), resulting in the extraction of reliable lattice parameters and the refinement of the molecular conformation at room temperature. The structure is hexagonal [space group P6(1)22, unit-cell parameters a = 71.0862 (3), c = 85.0276 (5) A] with 12 symmetry-related molecules in the unit cell, in agreement with previous studies. The results of our analysis are indicative of specific amino acids being disordered at this temperature. Upon cooling, a sudden drop in the lattice parameters at approximately 250 K is observed concurrently with the freezing of the mother liquor. The observation of severe peak broadening below this temperature indicates strain effects accompanying the freezing transition, which are found to be reversible. Finally, a correlation between the unit-cell parameters and the pH of the buffer solution is evident, in a similar manner to earlier observations on HEWL.
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The line below this paragraph, {{ABSTRACT_PUBMED_15805597}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15805597 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15805597}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Synchrotron X-ray powder diffraction study of hexagonal turkey egg-white lysozyme., Margiolaki I, Wright JP, Fitch AN, Fox GC, Von Dreele RB, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):423-32. Epub 2005, Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15805597 15805597]
Synchrotron X-ray powder diffraction study of hexagonal turkey egg-white lysozyme., Margiolaki I, Wright JP, Fitch AN, Fox GC, Von Dreele RB, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):423-32. Epub 2005, Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15805597 15805597]
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Binding of N-acetylglucosamine to chicken egg lysozyme: a powder diffraction study., Von Dreele RB, Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1836-42. Epub 2001, Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11717496 11717496]
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Structure of hexagonal turkey egg-white lysozyme at 1.65A resolution., Howell PL, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):654-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299795 15299795]
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X-ray structure of monoclinic turkey egg lysozyme at 1.3 A resolution., Harata K, Acta Crystallogr D Biol Crystallogr. 1993 Sep 1;49(Pt 5):497-504. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299509 15299509]
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Structure determination of turkey egg-white lysozyme using Laue diffraction data., Howell PL, Almo SC, Parsons MR, Hajdu J, Petsko GA, Acta Crystallogr B. 1992 Apr 1;48 ( Pt 2):200-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1515108 1515108]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
[[Category: Meleagris gallopavo]]
[[Category: Meleagris gallopavo]]
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[[Category: Powder diffraction]]
[[Category: Powder diffraction]]
[[Category: X-ray]]
[[Category: X-ray]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:58:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:30:02 2008''

Revision as of 04:30, 29 July 2008

Image:1xft.png

Template:STRUCTURE 1xft

Synchrotron X-ray Powder Diffraction Study of Hexagonal Turkey Egg-white Lysozyme

Template:ABSTRACT PUBMED 15805597

About this Structure

1XFT is a Single protein structure of sequence from Meleagris gallopavo. Full crystallographic information is available from OCA.

Reference

Synchrotron X-ray powder diffraction study of hexagonal turkey egg-white lysozyme., Margiolaki I, Wright JP, Fitch AN, Fox GC, Von Dreele RB, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):423-32. Epub 2005, Mar 24. PMID:15805597

Binding of N-acetylglucosamine to chicken egg lysozyme: a powder diffraction study., Von Dreele RB, Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1836-42. Epub 2001, Nov 21. PMID:11717496

Structure of hexagonal turkey egg-white lysozyme at 1.65A resolution., Howell PL, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):654-62. PMID:15299795

X-ray structure of monoclinic turkey egg lysozyme at 1.3 A resolution., Harata K, Acta Crystallogr D Biol Crystallogr. 1993 Sep 1;49(Pt 5):497-504. PMID:15299509

Structure determination of turkey egg-white lysozyme using Laue diffraction data., Howell PL, Almo SC, Parsons MR, Hajdu J, Petsko GA, Acta Crystallogr B. 1992 Apr 1;48 ( Pt 2):200-7. PMID:1515108

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