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2ffv

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{{STRUCTURE_2ffv| PDB=2ffv | SCENE= }}
{{STRUCTURE_2ffv| PDB=2ffv | SCENE= }}
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'''Human ppGalNAcT-2 complexed with manganese and UDP'''
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===Human ppGalNAcT-2 complexed with manganese and UDP===
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==Overview==
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The family of UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferases (ppGalNAcTs) is unique among glycosyltransferases, containing both catalytic and lectin domains that we have previously shown to be closely associated. Here we describe the x-ray crystal structures of human ppGalNAcT-2 (hT2) bound to the product UDP at 2.75 A resolution and to UDP and an acceptor peptide substrate EA2 (PTTDSTTPAPTTK) at 1.64 A resolution. The conformations of both UDP and residues Arg362-Ser372 vary greatly between the two structures. In the hT2-UDP-EA2 complex, residues Arg362-Ser373 comprise a loop that forms a lid over UDP, sealing it in the active site, whereas in the hT2-UDP complex this loop is folded back, exposing UDP to bulk solvent. EA2 binds in a shallow groove with threonine 7 positioned consistent with in vitro data showing it to be the preferred site of glycosylation. The relative orientations of the hT2 catalytic and lectin domains differ dramatically from that of murine ppGalNAcT-1 and also vary considerably between the two hT2 complexes. Indeed, in the hT2-UDP-EA2 complex essentially no contact is made between the catalytic and lectin domains except for the peptide bridge between them. Thus, the hT2 structures reveal an unexpected flexibility between the catalytic and lectin domains and suggest a new mechanism used by hT2 to capture glycosylated substrates. Kinetic analysis of hT2 lacking the lectin domain confirmed the importance of this domain in acting on glycopeptide but not peptide substrates. The structure of the hT2-UDP-EA2 complex also resolves long standing questions regarding ppGalNAcT acceptor substrate specificity.
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{{ABSTRACT_PUBMED_16434399}}
==About this Structure==
==About this Structure==
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[[Category: Mucin]]
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[[Category: Ppgalnact]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:50:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:33:15 2008''

Revision as of 04:33, 29 July 2008

Image:2ffv.png

Template:STRUCTURE 2ffv

Human ppGalNAcT-2 complexed with manganese and UDP

Template:ABSTRACT PUBMED 16434399

About this Structure

2FFV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Dynamic association between the catalytic and lectin domains of human UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase-2., Fritz TA, Raman J, Tabak LA, J Biol Chem. 2006 Mar 31;281(13):8613-9. Epub 2006 Jan 24. PMID:16434399

Page seeded by OCA on Tue Jul 29 07:33:15 2008

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