1tth

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{{STRUCTURE_1tth| PDB=1tth | SCENE= }}
{{STRUCTURE_1tth| PDB=1tth | SCENE= }}
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'''Aspartate Transcarbamoylase Catalytic Chain Mutant Glu50Ala Complexed with N-(Phosphonacetyl-L-Aspartate) (PALA)'''
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===Aspartate Transcarbamoylase Catalytic Chain Mutant Glu50Ala Complexed with N-(Phosphonacetyl-L-Aspartate) (PALA)===
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==Overview==
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A detailed description of the transition that allosteric enzymes undergo constitutes a major challenge in structural biology. We have succeeded in trapping four distinct allosteric states of a mutant enzyme of Escherichia coli aspartate transcarbomylase and determining their structures by X-ray crystallography. The mutant version of aspartate transcarbamoylase in which Glu50 in the catalytic chains was replaced by Ala destabilizes the native R state and shifts the equilibrium towards the T state. This behavior allowed the use of substrate analogs such as phosphonoacetamide and malonate to trap the enzyme in T-like and R-like structures that are distinct from the T-state structure of the wild-type enzyme (as represented by the structure of the enzyme with CTP bound and the R-state structure as represented by the structure with N-(phosphonacetyl)-L-aspartate bound). These structures shed light on the nature and the order of internal structural rearrangements during the transition from the T to the R state. They also suggest an explanation for diminished activity of the E50A enzyme and for the change in reaction mechanism from ordered to random for this mutant enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 15288791 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15288791}}
==About this Structure==
==About this Structure==
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[[Category: Domain closure]]
[[Category: Domain closure]]
[[Category: Site-specific mutagenesis]]
[[Category: Site-specific mutagenesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:20:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:39:06 2008''

Revision as of 04:39, 29 July 2008

Image:1tth.png

Template:STRUCTURE 1tth

Aspartate Transcarbamoylase Catalytic Chain Mutant Glu50Ala Complexed with N-(Phosphonacetyl-L-Aspartate) (PALA)

Template:ABSTRACT PUBMED 15288791

About this Structure

1TTH is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Monitoring the transition from the T to the R state in E.coli aspartate transcarbamoylase by X-ray crystallography: crystal structures of the E50A mutant enzyme in four distinct allosteric states., Stieglitz K, Stec B, Baker DP, Kantrowitz ER, J Mol Biol. 2004 Aug 13;341(3):853-68. PMID:15288791

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