2iv2

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{{STRUCTURE_2iv2| PDB=2iv2 | SCENE= }}
{{STRUCTURE_2iv2| PDB=2iv2 | SCENE= }}
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'''REINTERPRETATION OF REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''
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===REINTERPRETATION OF REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI===
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==Overview==
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Re-evaluation of the crystallographic data of the molybdenum-containing E. coli formate dehydrogenase H (Boyington et al. Science 275:1305-1308, 1997), reported in two redox states, reveals important structural differences for the formate-reduced form, with large implications for the reaction mechanism proposed in that work. We have re-refined the reduced structure with revised protocols and found substantial rearrangement in some parts of it. The original model is essentially correct but an important loop close to the molybdenum active site was mistraced, and, therefore, catalytic relevant residues were located in wrong positions. In particular selenocysteine-140, a ligand of molybdenum in the original work, and essential for catalysis, is no longer bound to the metal after reduction of the enzyme with formate. These results are incompatible with the originally proposed reaction mechanism. On the basis of our new interpretation, we have revised and proposed a new reaction mechanism, which reconciles the new X-ray model with previous biochemical and extended X-ray absorption fine structure data.
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{{ABSTRACT_PUBMED_16830149}}
==About this Structure==
==About this Structure==
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[[Category: Selenium]]
[[Category: Selenium]]
[[Category: Selenocysteine]]
[[Category: Selenocysteine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:55:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:50:45 2008''

Revision as of 04:50, 29 July 2008

Image:2iv2.png

Template:STRUCTURE 2iv2

REINTERPRETATION OF REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI

Template:ABSTRACT PUBMED 16830149

About this Structure

2IV2 is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Formate-reduced E. coli formate dehydrogenase H: The reinterpretation of the crystal structure suggests a new reaction mechanism., Raaijmakers HC, Romao MJ, J Biol Inorg Chem. 2006 Oct;11(7):849-54. Epub 2006 Jul 8. PMID:16830149

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