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1okh

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{{STRUCTURE_1okh| PDB=1okh | SCENE= }}
{{STRUCTURE_1okh| PDB=1okh | SCENE= }}
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'''VISCOTOXIN A3 FROM VISCUM ALBUM L.'''
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===VISCOTOXIN A3 FROM VISCUM ALBUM L.===
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==Overview==
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The crystal structure of viscotoxin A3 (VT A3) extracted from European mistletoe (Viscum album L.) has been solved using the anomalous diffraction of the native S atoms measured in-house with Cu Kalpha radiation to a resolution of 2.2 A and truncated to 2.5 A. A 1.75 A resolution synchrotron data set was used for phase expansion and refinement. An innovation in the dual-space substructure-solution program SHELXD enabled the individual S atoms of the disulfide bonds to be located using the Cu Kalpha data; this resulted in a marked improvement in the phasing compared with the use of super-S atoms. The VT A3 monomer consists of 46 amino acids with three disulfide bridges and has an overall fold resembling the canonical architecture of the alpha- and beta-thionins, a capital letter L. The asymmetric unit consists of two monomers related by a local twofold axis and held together by hydrophobic interactions between the monomer units. One phosphate anion (confirmed by 31P-NMR and MS) is associated with each monomer.
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(as it appears on PubMed at http://www.pubmed.gov), where 14646070 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14646070}}
==About this Structure==
==About this Structure==
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[[Category: Thionin]]
[[Category: Thionin]]
[[Category: Toxin]]
[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:58:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:54:34 2008''

Revision as of 04:54, 29 July 2008

Image:1okh.png

Template:STRUCTURE 1okh

VISCOTOXIN A3 FROM VISCUM ALBUM L.

Template:ABSTRACT PUBMED 14646070

About this Structure

1OKH is a Single protein structure of sequence from Viscum album. Full crystallographic information is available from OCA.

Reference

Structure of viscotoxin A3: disulfide location from weak SAD data., Debreczeni JE, Girmann B, Zeeck A, Kratzner R, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2125-32. Epub 2003, Nov 27. PMID:14646070

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