1t6h

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{{STRUCTURE_1t6h| PDB=1t6h | SCENE= }}
{{STRUCTURE_1t6h| PDB=1t6h | SCENE= }}
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'''Crystal Structure T4 Lysozyme incorporating an unnatural amino acid p-iodo-L-phenylalanine at position 153'''
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===Crystal Structure T4 Lysozyme incorporating an unnatural amino acid p-iodo-L-phenylalanine at position 153===
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==Overview==
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A recently developed method makes it possible to genetically encode unnatural amino acids with diverse physical, chemical or biological properties in Escherichia coli and yeast. We now show that this technology can be used to efficiently and site-specifically incorporate p-iodo-L-phenylalanine (iodoPhe) into proteins in response to an amber TAG codon. The selective introduction of the anomalously scattering iodine atom into proteins should facilitate single-wavelength anomalous dispersion experiments on in-house X-ray sources. To illustrate this, we generated a Phe153 --&gt; iodoPhe mutant of bacteriophage T4 lysozyme and determined its crystal structure using considerably less data than are needed for the equivalent experiment with cysteine and methionine. The iodoPhe residue, although present in the hydrophobic core of the protein, did not perturb the protein structure in any meaningful way. The ability to selectively introduce this and other heavy atom-containing amino acids into proteins should facilitate the structural study of proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 15378068 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15378068}}
==About this Structure==
==About this Structure==
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[[Category: Sad phasing]]
[[Category: Sad phasing]]
[[Category: Unnatural amino acid]]
[[Category: Unnatural amino acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:35:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:55:21 2008''

Revision as of 04:55, 29 July 2008

Image:1t6h.png

Template:STRUCTURE 1t6h

Crystal Structure T4 Lysozyme incorporating an unnatural amino acid p-iodo-L-phenylalanine at position 153

Template:ABSTRACT PUBMED 15378068

About this Structure

1T6H is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination., Xie J, Wang L, Wu N, Brock A, Spraggon G, Schultz PG, Nat Biotechnol. 2004 Oct;22(10):1297-301. Epub 2004 Sep 19. PMID:15378068

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