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| - | [[Image:1p7p.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1p7p| PDB=1p7p | SCENE= }} | | {{STRUCTURE_1p7p| PDB=1p7p | SCENE= }} |
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| - | '''Methionyl-tRNA synthetase from Escherichia coli complexed with methionine phosphonate'''
| + | ===Methionyl-tRNA synthetase from Escherichia coli complexed with methionine phosphonate=== |
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| - | ==Overview==
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| - | Binding of methionine to methionyl-tRNA synthetase (MetRS) is known to promote conformational changes within the active site. However, the contribution of these rearrangements to enzyme catalysis is not fully understood. In this study, several methionine and methionyl adenylate analogues were diffused into crystals of the monomeric form of Escherichia coli methionyl-tRNA synthetase. The structures of the corresponding complexes were solved at resolutions below 1.9A and compared to those of the enzyme free or complexed with methionine. Residues Y15 and W253 play key roles in the strength of the binding of the amino acid and of its analogues. Indeed, full motions of these residues are required to recover the maximum in free energy of binding. Residue Y15 also controls the size of the hydrophobic pocket where the amino acid side-chain interacts. H301 appears to participate to the specific recognition of the sulphur atom of methionine. Complexes with methionyl adenylate analogues illustrate the shielding by MetRS of the region joining the methionine and adenosine moieties. Finally, the structure of MetRS complexed to a methionine analogue mimicking the tetrahedral carbon of the transition state in the aminoacylation reaction was solved. On the basis of this model, we propose that, in response to the binding of the 3'-end of tRNA, Y15 moves again in order to deshield the anhydride bond in the natural adenylate.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12946347}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12946347 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12946347}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Ligase]] | | [[Category: Ligase]] |
| | [[Category: Rossmann fold]] | | [[Category: Rossmann fold]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:47:20 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:01:44 2008'' |
Revision as of 05:01, 29 July 2008
Template:STRUCTURE 1p7p
Methionyl-tRNA synthetase from Escherichia coli complexed with methionine phosphonate
Template:ABSTRACT PUBMED 12946347
About this Structure
1P7P is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Use of analogues of methionine and methionyl adenylate to sample conformational changes during catalysis in Escherichia coli methionyl-tRNA synthetase., Crepin T, Schmitt E, Mechulam Y, Sampson PB, Vaughan MD, Honek JF, Blanquet S, J Mol Biol. 2003 Sep 5;332(1):59-72. PMID:12946347
Page seeded by OCA on Tue Jul 29 08:01:44 2008
