1nce

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{{STRUCTURE_1nce| PDB=1nce | SCENE= }}
{{STRUCTURE_1nce| PDB=1nce | SCENE= }}
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'''Crystal structure of a ternary complex of E. coli thymidylate synthase D169C with dUMP and the antifolate CB3717'''
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===Crystal structure of a ternary complex of E. coli thymidylate synthase D169C with dUMP and the antifolate CB3717===
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==Overview==
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Cysteine is the only variant of D169, a cofactor-binding residue in thymidylate synthase, that shows in vivo activity. The 2.4 A crystal structure of Escherichia coli thymidylate synthase D169C in a complex with dUMP and the antifolate CB3717 shows it to be an asymmetric dimer, with only one active site covalently bonded to dUMP. At the active site with covalently bound substrate, C169 S gamma adopts the roles of both carboxyl oxygens of D169, making a 3.6 A S...H[bond]N hydrogen bond to 3-NH of CB3717 and a 3.4 A water-mediated hydrogen bond to H212. Analogous hydrogen bonds formed during the enzyme reaction are important for cofactor binding and are postulated to contribute to catalysis. The C169 side chain is likely to be ionized, making it a better hydrogen bond acceptor than a neutral sulfhydryl group. At the second active site, C169 S gamma makes a shorter (3 A) hydrogen bond to the 3-NH of CB3717, CB3717 is approximately 1.5 A out of its binding site and there is no covalent bond between dUMP and the catalytic cysteine. Changes to partitioning among productive and non-productive conformations of reaction intermediates may contribute as much, if not more, to the diminished activity of this mutant than reduced stabilization of transition states.
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(as it appears on PubMed at http://www.pubmed.gov), where 12702803 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12702803}}
==About this Structure==
==About this Structure==
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[[Category: Beta-sheet interface]]
[[Category: Beta-sheet interface]]
[[Category: Protein-dump-cofactor analog complex]]
[[Category: Protein-dump-cofactor analog complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:22:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:08:58 2008''

Revision as of 05:09, 29 July 2008

Image:1nce.png

Template:STRUCTURE 1nce

Crystal structure of a ternary complex of E. coli thymidylate synthase D169C with dUMP and the antifolate CB3717

Template:ABSTRACT PUBMED 12702803

About this Structure

1NCE is a Single protein structure of sequence from Escherichia coli, and escherichia coli o157:h7. Full crystallographic information is available from OCA.

Reference

The only active mutant of thymidylate synthase D169, a residue far from the site of methyl transfer, demonstrates the exquisite nature of enzyme specificity., Birdsall DL, Finer-Moore J, Stroud RM, Protein Eng. 2003 Mar;16(3):229-40. PMID:12702803

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