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2p1d

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{{STRUCTURE_2p1d| PDB=2p1d | SCENE= }}
{{STRUCTURE_2p1d| PDB=2p1d | SCENE= }}
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'''Crystal structure of dengue methyltransferase in complex with GTP and S-Adenosyl-L-homocysteine'''
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===Crystal structure of dengue methyltransferase in complex with GTP and S-Adenosyl-L-homocysteine===
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==Overview==
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Viruses represent an attractive system with which to study the molecular basis of mRNA capping and its relation to the RNA transcription machinery. The RNA-dependent RNA polymerase NS5 of flaviviruses presents a characteristic motif of S-adenosyl-L-methionine-dependent methyltransferases at its N-terminus, and polymerase motifs at its C-terminus. The crystal structure of an N-terminal fragment of Dengue virus type 2 NS5 is reported at 2.4 A resolution. We show that this NS5 domain includes a typical methyltransferase core and exhibits a (nucleoside-2'-O-)-methyltransferase activity on capped RNA. The structure of a ternary complex comprising S-adenosyl-L-homocysteine and a guanosine triphosphate (GTP) analogue shows that 54 amino acids N-terminal to the core provide a novel GTP-binding site that selects guanine using a previously unreported mechanism. Binding studies using GTP- and RNA cap-analogues, as well as the spatial arrangement of the methyltransferase active site relative to the GTP-binding site, suggest that the latter is a specific cap-binding site. As RNA capping is an essential viral function, these results provide a structural basis for the rational design of drugs against the emerging flaviviruses.
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(as it appears on PubMed at http://www.pubmed.gov), where 12032088 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12032088}}
==About this Structure==
==About this Structure==
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[[Category: Vizier]]
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[[Category: Vizier. viral enzymes involved in replication]]
[[Category: Vizier. viral enzymes involved in replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:08:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:15:02 2008''

Revision as of 05:15, 29 July 2008

Image:2p1d.png

Template:STRUCTURE 2p1d

Crystal structure of dengue methyltransferase in complex with GTP and S-Adenosyl-L-homocysteine

Template:ABSTRACT PUBMED 12032088

About this Structure

2P1D is a Single protein structure of sequence from Dengue virus 2. Full crystallographic information is available from OCA.

Reference

An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization., Egloff MP, Benarroch D, Selisko B, Romette JL, Canard B, EMBO J. 2002 Jun 3;21(11):2757-68. PMID:12032088

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