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| - | [[Image:1x8f.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1x8f| PDB=1x8f | SCENE= }} | | {{STRUCTURE_1x8f| PDB=1x8f | SCENE= }} |
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| - | '''Crystal Structure Of apo-Kdo8P Synthase'''
| + | ===Crystal Structure Of apo-Kdo8P Synthase=== |
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| - | ==Overview==
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| - | The enzyme 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8PS) catalyses the condensation of arabinose 5-phosphate (A5P) and phosphoenol pyruvate (PEP) to obtain 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P). We have elucidated initial modes of ligand binding in KDO8PS binary complexes by X-ray crystallography. Structures of the apo-enzyme and of binary complexes with the substrate PEP, the product KDO8P and the catalytically inactive 1-deoxy analog of arabinose 5-phosphate (1dA5P) were obtained. The KDO8PS active site resembles an irregular funnel with positive electrostatic potential situated at the bottom of the PEP-binding sub-site, which is the primary attractive force towards negatively charged phosphate moieties of all ligands. The structures of the ligand-free apo-KDO8PS and the binary complex with the product KDO8P visualize for the first time the role of His202 as an active-site gate. Examination of the crystal structures of KDO8PS with the KDO8P or 1dA5P shows these ligands bound to the enzyme in the PEP-binding sub-site, and not as expected to the A5P sub-site. Taken together, the structures presented here strengthen earlier evidence that this enzyme functions predominantly through positional catalysis, map out the roles of active-site residues and provide evidence that explains the total lack of catalytic reversibility. | + | The line below this paragraph, {{ABSTRACT_PUBMED_16023668}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16023668 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16023668}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Lipopolysaccharide]] | | [[Category: Lipopolysaccharide]] |
| | [[Category: Lyase]] | | [[Category: Lyase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:42:06 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:18:41 2008'' |
Revision as of 05:18, 29 July 2008
Template:STRUCTURE 1x8f
Crystal Structure Of apo-Kdo8P Synthase
Template:ABSTRACT PUBMED 16023668
About this Structure
1X8F is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structures of Escherichia coli KDO8P synthase complexes reveal the source of catalytic irreversibility., Vainer R, Belakhov V, Rabkin E, Baasov T, Adir N, J Mol Biol. 2005 Aug 19;351(3):641-52. PMID:16023668
Page seeded by OCA on Tue Jul 29 08:18:41 2008
