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2jub

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[[Image:2jub.jpg|left|200px]]
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[[Image:2jub.png|left|200px]]
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{{STRUCTURE_2jub| PDB=2jub | SCENE= }}
{{STRUCTURE_2jub| PDB=2jub | SCENE= }}
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'''Solution structure of IPI*'''
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===Solution structure of IPI*===
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==Overview==
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Phage T4 protects its DNA from the two-gene-encoded gmrS/gmrD (glucose-modified hydroxymethylcytosine restriction endonuclease) CT of pathogenic Escherichia coli, CT596, by injecting several hundred copies of the 76-amino-acid-residue nuclease inhibitor, IPI*, into the infected host. Here, the three-dimensional solution structure of mature IPI* is reported as determined by nuclear magnetic resonance techniques using 1290 experimental nuclear Overhauser effect and dipolar coupling constraints ( approximately 17 constraints per residue). Close examination of this oblate-shaped protein structure reveals a novel fold consisting of two small beta-sheets (beta1: B1 and B2; beta2: B3-B5) flanked at the N- and C-termini by alpha-helices (H1 and H2). Such a fold is very compact in shape and allows ejection of IPI* through the narrow 30-A portal and tail tube apertures of the virion without unfolding. Structural and dynamic measurements identify an exposed hydrophobic knob that is a putative gmrS/gmrD-binding site. A single gene from the uropathogenic E. coli UT189, which codes for a gmrS/gmrD-like UT fusion enzyme (with approximately 90% identity to the heterodimeric CT enzyme), has evolved IPI* inhibitor immunity. Analysis of the gmrS/gmrD restriction endonuclease enzyme family and its IPI* family phage antagonists reveals an evolutionary pathway that has elaborated a surprisingly diverse and specifically fitted set of coevolving attack and defense structures.
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(as it appears on PubMed at http://www.pubmed.gov), where 18037438 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18037438}}
==About this Structure==
==About this Structure==
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2JUB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUB OCA].
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2JUB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUB OCA].
==Reference==
==Reference==
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[[Category: Solution]]
[[Category: Solution]]
[[Category: T4 phage]]
[[Category: T4 phage]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:17:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:19:38 2008''

Revision as of 05:19, 29 July 2008

Image:2jub.png

Template:STRUCTURE 2jub

Solution structure of IPI*

Template:ABSTRACT PUBMED 18037438

About this Structure

2JUB is a Single protein structure of sequence from Enterobacteria phage t4. Full experimental information is available from OCA.

Reference

Restriction endonuclease inhibitor IPI* of bacteriophage T4: a novel structure for a dedicated target., Rifat D, Wright NT, Varney KM, Weber DJ, Black LW, J Mol Biol. 2008 Jan 18;375(3):720-34. Epub 2007 Nov 1. PMID:18037438

Page seeded by OCA on Tue Jul 29 08:19:38 2008

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