2z4g

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{{STRUCTURE_2z4g| PDB=2z4g | SCENE= }}
{{STRUCTURE_2z4g| PDB=2z4g | SCENE= }}
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'''Histidinol Phosphate Phosphatase from Thermus thermophilus HB8'''
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===Histidinol Phosphate Phosphatase from Thermus thermophilus HB8===
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==Overview==
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Monofunctional histidinol phosphate phosphatase from Thermus thermophilus HB8, which catalyzes the dephosphorylation of l-histidinol phosphate, belongs to the PHP family, together with the PHP domain of bacterial DNA polymerase III and family X DNA polymerase. We have determined the structures of the complex with a sulfate ion, the complex with a phosphate ion, and the unliganded form at 1.6, 2.1, and 1.8 A resolution, respectively. The enzyme exists as a tetramer, and the subunit consists of a distorted (betaalpha)7 barrel with one linker and one C-terminal tail. Three metal sites located on the C-terminal side of the barrel are occupied by Fe1, Fe2, and Zn ions, respectively, forming a trinuclear metal center liganded by seven histidines, one aspartate, one glutamate, and one hydroxide with two Fe ions bridged by the hydroxide. In the complexes, the sulfate or phosphate ion is coordinated to three metal ions, resulting in octahedral, trigonal bipyramidal, and tetrahedral geometries around the Fe1, Fe2, and Zn ions, respectively. The ligand residues are derived from the four motifs that characterize the PHP family and from two motifs conserved in histidinol phosphate phosphatases. The (betaalpha)7 barrel and the metal cluster are closely related in nature and architecture to the (betaalpha)8 barrel and the mononuclear or dinuclear metal center in the amidohydrolase superfamily, respectively. The coordination behavior of the phosphate ion toward the metal center supports the mechanism in which the bridging hydroxide makes a direct attack on the substrate phosphate tridentately bound to the two Fe ions and Zn ion to hydrolyze the phosphoester bond.
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(as it appears on PubMed at http://www.pubmed.gov), where 17929834 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17929834}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Metal-dependent]]
[[Category: Metal-dependent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:40:06 2008''

Revision as of 05:40, 29 July 2008

Image:2z4g.png

Template:STRUCTURE 2z4g

Histidinol Phosphate Phosphatase from Thermus thermophilus HB8

Template:ABSTRACT PUBMED 17929834

About this Structure

2Z4G is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Crystal structure of monofunctional histidinol phosphate phosphatase from Thermus thermophilus HB8., Omi R, Goto M, Miyahara I, Manzoku M, Ebihara A, Hirotsu K, Biochemistry. 2007 Nov 6;46(44):12618-27. Epub 2007 Oct 11. PMID:17929834

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