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1wl9

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{{STRUCTURE_1wl9| PDB=1wl9 | SCENE= }}
{{STRUCTURE_1wl9| PDB=1wl9 | SCENE= }}
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'''Structure of aminopeptidase P from E. coli'''
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===Structure of aminopeptidase P from E. coli===
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==Overview==
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The effect of metal substitution on the activity and structure of the aminopeptidase P (APPro) from Escherichia coli has been investigated. Measurements of activity in the presence of Mn2+, Mg2+, Zn2+, Na+, and Ca2+ show that significant activity is seen only in the Mn-bound form of the enzyme. The addition of Zn2+ to [MnMn(APPro)] is strongly inhibitory. Crystal structures of [MnMn(APPro)], [MgMg(APPro)], [ZnZn(APPro)], [ZnMg(APPro)], [Ca_(APPro)], [Na_(APPro)], and [apo(APPro)] were determined. The structures of [Ca_(APPro)] and [Na_(APPro)] have a single metal atom at their active site. Surprisingly, when a tripeptide substrate (ValProLeu) was soaked into [Na_(APPro)] crystals in the presence of 200 mM Mg2+, the structure had substrate, but no metal, bound at the active site. The structure of apo APPro complexed with ValProLeu shows that the N-terminal amino group of a substrate can be bound at the active site by carboxylate side chains that normally bind the second metal atom, providing a model for substrate binding in a single-metal active enzyme. Structures of [MnMn(APPro)] and [ZnZn(APPro)] complexes of ProLeu, a product inhibitor, in the presence of excess Zn reveal a third metal-binding site, formed by two conserved His residues and the dipeptide inhibitor. A Zn atom bound at such a site would stabilize product binding and enhance inhibition.
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{{ABSTRACT_PUBMED_16229471}}
==About this Structure==
==About this Structure==
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[[Category: Pita-bread fold]]
[[Category: Pita-bread fold]]
[[Category: Proline-specific peptidase]]
[[Category: Proline-specific peptidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:50:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:43:23 2008''

Revision as of 05:43, 29 July 2008

Image:1wl9.png

Template:STRUCTURE 1wl9

Structure of aminopeptidase P from E. coli

Template:ABSTRACT PUBMED 16229471

About this Structure

1WL9 is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 1az9. Full crystallographic information is available from OCA.

Reference

Structural and functional implications of metal ion selection in aminopeptidase P, a metalloprotease with a dinuclear metal center., Graham SC, Bond CS, Freeman HC, Guss JM, Biochemistry. 2005 Oct 25;44(42):13820-36. PMID:16229471

Page seeded by OCA on Tue Jul 29 08:43:23 2008

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