1png
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1png" size="450" color="white" frame="true" align="right" spinBox="true" caption="1png, resolution 2.2Å" /> '''CRYSTAL STRUCTURE OF ...)
Next diff →
Revision as of 21:49, 20 November 2007
|
CRYSTAL STRUCTURE OF PEPTIDE-N(4)-(N-ACETYL-BETA-D-GLUCOSAMINYL) ASPARAGINE AMIDASE AT 2.2 ANGSTROMS RESOLUTION
Overview
Peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F (PNGase F), is an amidase that cleaves the beta-aspartylglucosylamine bond of, asparagine-linked glycans. The 34.8-kDa (314 amino acids) enzyme has a, very broad substrate specificity and is extensively used for studies of, the structure and function of glycoproteins. Enzymatic activity of PNGase, F requires recognition of both the peptide and the carbohydrate components, of the substrate. Only limited information regarding the mechanism of, action of the enzyme is available. The three-dimensional structure of, PNGase F has been determined by X-ray crystallography at 2.2-A resolution., The protein folds into two domains comprising residues 1-137 and 143-314, respectively. Both domains have eight-stranded antiparallel beta-sandwich, motifs that are very similar in geometry. Both sandwiches have parallel, principal axes and lie side by side. The covalent link between the domains, is located at the top end of the molecule. Extensive hydrogen-bonding, contacts occur along the full length of the interface between the two, domains. Three different areas, all at the interface between the two, domains, have been identified as possible locations for the active site of, the enzyme. These include a hydrophobic bowl of about 20 A in diameter on, one surface of the molecule, a long polar cleft on the opposite side, and, a cleft at the bottom, which is lined with large aromatic residues, including eight tryptophans.
About this Structure
1PNG is a Single protein structure of sequence from Elizabethkingia meningoseptica. Active as Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase, with EC number 3.5.1.52 Full crystallographic information is available from OCA.
Reference
Crystal structure of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F at 2.2-A resolution., Kuhn P, Tarentino AL, Plummer TH Jr, Van Roey P, Biochemistry. 1994 Oct 4;33(39):11699-706. PMID:7918386
Page seeded by OCA on Tue Nov 20 23:56:48 2007
