2oqn

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{{Seed}}
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{{STRUCTURE_2oqn| PDB=2oqn | SCENE= }}
{{STRUCTURE_2oqn| PDB=2oqn | SCENE= }}
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'''High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths'''
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===High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths===
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==Overview==
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Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.
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(as it appears on PubMed at http://www.pubmed.gov), where 17452791 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17452791}}
==About this Structure==
==About this Structure==
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[[Category: High pressure cryocooling]]
[[Category: High pressure cryocooling]]
[[Category: Sulfur sad phasing]]
[[Category: Sulfur sad phasing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:28:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:01:50 2008''

Revision as of 06:01, 29 July 2008

Image:2oqn.png

Template:STRUCTURE 2oqn

High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths

Template:ABSTRACT PUBMED 17452791

About this Structure

2OQN is a Single protein structure of sequence from Thaumatococcus daniellii. Full crystallographic information is available from OCA.

Reference

High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths., Kim CU, Hao Q, Gruner SM, Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:17452791

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