1pop
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(New page: 200px<br /><applet load="1pop" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pop, resolution 2.1Å" /> '''X-RAY CRYSTALLOGRAPHI...)
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Revision as of 21:51, 20 November 2007
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X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX
Overview
The three-dimensional structure of the papain-leupeptin complex has been, determined by X-ray crystallography to a resolution of 2.1 A (overall, R-factor = 19.8%). The structure indicates that: (i) leupeptin contacts, the S subsites of the papain active site and not the S' subsites; (ii) the, 'carbonyl' carbon atom of the inhibitor is covalently bound by the Cys-25, sulphur atom of papain and is tetrahedrally coordinated; (iii) the, 'carbonyl' oxygen atom of the inhibitor faces the oxyanion hole and makes, hydrogen bond contacts with Gln-19 and Cys-25.
About this Structure
1POP is a Single protein structure of sequence from [1] with ACE and MOH as ligands. Active as Papain, with EC number 3.4.22.2 Full crystallographic information is available from OCA.
Reference
X-ray crystallographic structure of a papain-leupeptin complex., Schroder E, Phillips C, Garman E, Harlos K, Crawford C, FEBS Lett. 1993 Jan 2;315(1):38-42. PMID:8416808
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