1ujz

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[[Image:1ujz.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1ujz| PDB=1ujz | SCENE= }}
{{STRUCTURE_1ujz| PDB=1ujz | SCENE= }}
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'''Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein'''
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===Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein===
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==Overview==
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We developed a 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface and applied it to create new specifically interacting DNase-inhibitor protein pairs. We demonstrate that the designed switch in specificity holds in in vitro binding and functional assays. We also show that the designed interfaces are specific in the natural functional context in living cells, and present the first high-resolution X-ray crystallographic analysis of a computer-redesigned functional protein-protein interface with altered specificity. The approach should be applicable to the design of interacting protein pairs with novel specificities for delineating and re-engineering protein interaction networks in living cells.
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The line below this paragraph, {{ABSTRACT_PUBMED_15034550}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15034550 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15034550}}
==About this Structure==
==About this Structure==
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[[Category: Redesigned protein-protein interface]]
[[Category: Redesigned protein-protein interface]]
[[Category: Specificity]]
[[Category: Specificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:19:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:05:23 2008''

Revision as of 06:05, 29 July 2008

Image:1ujz.png

Template:STRUCTURE 1ujz

Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein

Template:ABSTRACT PUBMED 15034550

About this Structure

1UJZ is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Computational redesign of protein-protein interaction specificity., Kortemme T, Joachimiak LA, Bullock AN, Schuler AD, Stoddard BL, Baker D, Nat Struct Mol Biol. 2004 Apr;11(4):371-9. Epub 2004 Mar 21. PMID:15034550

Page seeded by OCA on Tue Jul 29 09:05:23 2008

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