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1xzo

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{{STRUCTURE_1xzo| PDB=1xzo | SCENE= }}
{{STRUCTURE_1xzo| PDB=1xzo | SCENE= }}
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'''Identification of a disulfide switch in BsSco, a member of the Sco family of cytochrome c oxidase assembly proteins'''
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===Identification of a disulfide switch in BsSco, a member of the Sco family of cytochrome c oxidase assembly proteins===
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==Overview==
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BsSco is a membrane-associated protein from Bacillus subtilis characterized by the sequence CXXXCP, which is conserved in yeast and human mitochondrial Sco proteins, and their bacterial homologues. BsSco is involved in the assembly of the Cu(A) center in cytochrome c oxidase and may play a role in the transfer of copper to this site. We have characterized the soluble domain of BsSco by biochemical, spectroscopic, and structural approaches. Soluble BsSco is monomeric in solution, and the two conserved cysteines are involved in an intramolecular cystine bridge. The cystine bridge is easily reduced, and circular dichroism spectroscopy shows no large-scale changes in BsSco's secondary structure upon reduction. The crystal structure of soluble BsSco, determined at 1.7 A resolution, reveals typical elements of a thioredoxin fold. The CXXXCP motif, in which Cys45 and Cys49 are conserved, is located in a turn structure on the surface of the protein. In various native and His135Ala mutant structures, both disulfide-bonded and non-disulfide-bonded forms of CXXXCP are observed. However, despite extensive attempts, copper has not been found near or beyond the CXXXCP motif, a presumptive copper-binding site. Another potential copper binding residue, His135, is located in a highly flexible loop parallel to the CXXXCP loop but is more than 10 A from Cys45 and Cys49. If these three residues are to coordinate copper, a conformational change is necessary. The structural identification of a disulfide switch demonstrates that BsSco has the capability to fill a redox role in Cu(A) assembly.
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{{ABSTRACT_PUBMED_15723536}}
==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Thioredoxin-like fold]]
[[Category: Thioredoxin-like fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:07:49 2008''

Revision as of 06:07, 29 July 2008

Image:1xzo.png

Template:STRUCTURE 1xzo

Identification of a disulfide switch in BsSco, a member of the Sco family of cytochrome c oxidase assembly proteins

Template:ABSTRACT PUBMED 15723536

About this Structure

1XZO is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Identification of a disulfide switch in BsSco, a member of the Sco family of cytochrome c oxidase assembly proteins., Ye Q, Imriskova-Sosova I, Hill BC, Jia Z, Biochemistry. 2005 Mar 1;44(8):2934-42. PMID:15723536

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