2juk

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[[Image:2juk.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2juk| PDB=2juk | SCENE= }}
{{STRUCTURE_2juk| PDB=2juk | SCENE= }}
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'''guanidino neomycin B recognition of an HIV-1 RNA helix'''
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===guanidino neomycin B recognition of an HIV-1 RNA helix===
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==Overview==
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Aminoglycoside antibiotics are small-molecule drugs that bind RNA. The affinity and specificity of aminoglycoside binding to RNA can be increased through chemical modification, such as guanidinylation. Here, we report the binding of guanidinoneomycin B (GNB) to an RNA helix from the HIV-1 frameshift site. The binding of GNB increases the melting temperature (T(m)) of the frameshift-site RNA by at least 10 degrees C, to a point at which a melting transition is not even observed in 2 M urea. A structure of the complex was obtained by using multidimensional heteronuclear NMR spectroscopic methods. We also used a novel paramagnetic-probe assay to identify the site of GNB binding to the surface of the RNA. GNB makes major-groove contacts to two sets of Watson-Crick bases and is in van der Waals contact with a highly structured ACAA tetraloop. Rings I and II of GNB fit into the major groove and form the binding interface with the RNA, whereas rings III and IV are exposed to the solvent and disordered. The binding of GNB causes a broadening of the major groove across the binding site.
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The line below this paragraph, {{ABSTRACT_PUBMED_18058789}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 18058789 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18058789}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUK OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUK OCA].
==Reference==
==Reference==
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[[Category: Rna]]
[[Category: Rna]]
[[Category: Rna-ligand interaction]]
[[Category: Rna-ligand interaction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:18:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:08:14 2008''

Revision as of 06:08, 29 July 2008

Image:2juk.png

Template:STRUCTURE 2juk

guanidino neomycin B recognition of an HIV-1 RNA helix

Template:ABSTRACT PUBMED 18058789

About this Structure

Full experimental information is available from OCA.

Reference

Guanidinoneomycin B recognition of an HIV-1 RNA helix., Staple DW, Venditti V, Niccolai N, Elson-Schwab L, Tor Y, Butcher SE, Chembiochem. 2008 Jan 4;9(1):93-102. PMID:18058789

Page seeded by OCA on Tue Jul 29 09:08:14 2008

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