This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2g3k

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2g3k.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2g3k.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2g3k| PDB=2g3k | SCENE= }}
{{STRUCTURE_2g3k| PDB=2g3k | SCENE= }}
-
'''Crystal structure of the C-terminal domain of Vps28'''
+
===Crystal structure of the C-terminal domain of Vps28===
-
==Overview==
+
<!--
-
The endosomal sorting complex I required for transport (ESCRT-I) is composed of the three subunits Vps23/Tsg101, Vps28 and Vps37. ESCRT-I is recruited to cellular membranes during multivesicular endosome biogenesis and by enveloped viruses such as HIV-1 to mediate budding from the cell. Here, we describe the crystal structure of a conserved C-terminal domain from Sacharomyces cerevisiae Vps28 (Vps28-CTD) at 3.05 A resolution which folds independently into a four-helical bundle structure. Co-expression experiments of Vps28-CTD, Vps23 and Vps37 suggest that Vps28-CTD does not directly participate in ESCRT-I assembly and may thus act as an adaptor module for downstream interaction partners. We show through mutagenesis studies that Vps28-CTD employs its strictly conserved surface in the interaction with the ESCRT-III factor Vps20. Furthermore, we present evidence that Vps28-CTD is sufficient to rescue an equine infectious anaemia virus (EIAV) Gag late domain deletion. Vps28-CTD mutations abolishing Vps20 interaction in vitro also prevent the rescue of the EIAV Gag late domain mutant consistent with a potential direct Vps28-ESCRT-III Vps20 recruitment. Therefore, the physiological relevant EIAV Gag-Alix interaction can be functionally replaced by a Gag-Vps28-CTD fusion. Because both Alix and Vps28-CTD can directly recruit ESCRT-III proteins, ESCRT-III assembly coupled to Vps4 action may therefore constitute the minimal budding machinery for EIAV release.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16749904}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16749904 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16749904}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Weissenhorn, W.]]
[[Category: Weissenhorn, W.]]
[[Category: 4 helix bundle]]
[[Category: 4 helix bundle]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:39:01 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:14:51 2008''

Revision as of 06:14, 29 July 2008

Image:2g3k.png

Template:STRUCTURE 2g3k

Crystal structure of the C-terminal domain of Vps28

Template:ABSTRACT PUBMED 16749904

About this Structure

2G3K is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

The crystal structure of the C-terminal domain of Vps28 reveals a conserved surface required for Vps20 recruitment., Pineda-Molina E, Belrhali H, Piefer AJ, Akula I, Bates P, Weissenhorn W, Traffic. 2006 Aug;7(8):1007-16. Epub 2006 Jun 2. PMID:16749904

Page seeded by OCA on Tue Jul 29 09:14:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2g3k"
Personal tools