We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2g3k

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2g3k.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2g3k.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2g3k| PDB=2g3k | SCENE= }}
{{STRUCTURE_2g3k| PDB=2g3k | SCENE= }}
-
'''Crystal structure of the C-terminal domain of Vps28'''
+
===Crystal structure of the C-terminal domain of Vps28===
-
==Overview==
+
<!--
-
The endosomal sorting complex I required for transport (ESCRT-I) is composed of the three subunits Vps23/Tsg101, Vps28 and Vps37. ESCRT-I is recruited to cellular membranes during multivesicular endosome biogenesis and by enveloped viruses such as HIV-1 to mediate budding from the cell. Here, we describe the crystal structure of a conserved C-terminal domain from Sacharomyces cerevisiae Vps28 (Vps28-CTD) at 3.05 A resolution which folds independently into a four-helical bundle structure. Co-expression experiments of Vps28-CTD, Vps23 and Vps37 suggest that Vps28-CTD does not directly participate in ESCRT-I assembly and may thus act as an adaptor module for downstream interaction partners. We show through mutagenesis studies that Vps28-CTD employs its strictly conserved surface in the interaction with the ESCRT-III factor Vps20. Furthermore, we present evidence that Vps28-CTD is sufficient to rescue an equine infectious anaemia virus (EIAV) Gag late domain deletion. Vps28-CTD mutations abolishing Vps20 interaction in vitro also prevent the rescue of the EIAV Gag late domain mutant consistent with a potential direct Vps28-ESCRT-III Vps20 recruitment. Therefore, the physiological relevant EIAV Gag-Alix interaction can be functionally replaced by a Gag-Vps28-CTD fusion. Because both Alix and Vps28-CTD can directly recruit ESCRT-III proteins, ESCRT-III assembly coupled to Vps4 action may therefore constitute the minimal budding machinery for EIAV release.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16749904}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16749904 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16749904}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Weissenhorn, W.]]
[[Category: Weissenhorn, W.]]
[[Category: 4 helix bundle]]
[[Category: 4 helix bundle]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:39:01 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:14:51 2008''

Revision as of 06:14, 29 July 2008

Image:2g3k.png

Template:STRUCTURE 2g3k

Crystal structure of the C-terminal domain of Vps28

Template:ABSTRACT PUBMED 16749904

About this Structure

2G3K is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

The crystal structure of the C-terminal domain of Vps28 reveals a conserved surface required for Vps20 recruitment., Pineda-Molina E, Belrhali H, Piefer AJ, Akula I, Bates P, Weissenhorn W, Traffic. 2006 Aug;7(8):1007-16. Epub 2006 Jun 2. PMID:16749904

Page seeded by OCA on Tue Jul 29 09:14:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2g3k"
Personal tools