2fhz

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{{STRUCTURE_2fhz| PDB=2fhz | SCENE= }}
{{STRUCTURE_2fhz| PDB=2fhz | SCENE= }}
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'''Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein'''
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===Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein===
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==Overview==
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Colicin E5 is a tRNA-specific ribonuclease that recognizes and cleaves four tRNAs in Escherichia coli that contain the hypermodified nucleoside queuosine (Q) at the wobble position. Cells that produce colicin E5 also synthesize the cognate immunity protein (Im5) that rapidly and tightly associates with colicin E5 to prevent it from cleaving its own tRNAs to avoid suicide. We report here the crystal structure of Im5 in a complex with the activity domain of colicin E5 (E5-CRD) at 1.15A resolution. The structure reveals an extruded domain from Im5 that docks into the recessed RNA binding cleft in E5-CRD, resulting in extensive interactions between the two proteins. The interactions are primarily hydrophilic, with an interface that contains complementary surface charges between the two proteins. Detailed interactions in three separate regions of the interface account for specific recognition of colicin E5 by Im5. Furthermore, single-site mutational studies of Im5 confirmed the important role of particular residues in recognition and binding of colicin E5. Structural comparison of the complex reported here with E5-CRD alone, as well as with a docking model of RNA-E5-CRD, indicates that Im5 achieves its inhibition by physically blocking the cleft in colicin E5 that engages the RNA substrate.
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The line below this paragraph, {{ABSTRACT_PUBMED_16524591}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16524591 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16524591}}
==About this Structure==
==About this Structure==
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[[Category: Inhibition of ribonuclease]]
[[Category: Inhibition of ribonuclease]]
[[Category: Protein-protein complex]]
[[Category: Protein-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:55:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:18:33 2008''

Revision as of 06:18, 29 July 2008

Image:2fhz.png

Template:STRUCTURE 2fhz

Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein

Template:ABSTRACT PUBMED 16524591

About this Structure

2FHZ is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Molecular basis of inhibition of the ribonuclease activity in colicin E5 by its cognate immunity protein., Luna-Chavez C, Lin YL, Huang RH, J Mol Biol. 2006 Apr 28;358(2):571-9. Epub 2006 Feb 24. PMID:16524591

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