1oxw

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[[Image:1oxw.gif|left|200px]]
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{{Seed}}
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[[Image:1oxw.png|left|200px]]
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{{STRUCTURE_1oxw| PDB=1oxw | SCENE= }}
{{STRUCTURE_1oxw| PDB=1oxw | SCENE= }}
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'''The Crystal Structure of SeMet Patatin'''
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===The Crystal Structure of SeMet Patatin===
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==Overview==
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Patatin is a nonspecific lipid acyl hydrolase that accounts for approximately 40% of the total soluble protein in mature potato tubers, and it has potent insecticidal activity against the corn rootworm. We determined the X-ray crystal structure of a His-tagged variant of an isozyme of patatin, Pat17, to 2.2 A resolution, employing SeMet multiwavelength anomalous dispersion (MAD) phasing methods. The patatin crystal structure has three molecules in the asymmetric unit, an R-factor of 22.0%, and an R(free) of 27.2% (for 10% of the data not included in the refinement) and includes 498 water molecules. The structure notably revealed that patatin has a Ser-Asp catalytic dyad and an active site like that of human cytosolic phospholipase A(2) (cPLA(2)) [Dessen, A., et al. (1999) Cell 97, 349-360]. In addition, patatin has a folding topology related to that of the catalytic domain of cPLA(2) and unlike the canonical alpha/beta-hydrolase fold. The structure confirms our site-directed mutagenesis and bioactivity data that initially suggested patatin possessed a Ser-Asp catalytic dyad. Alanine-scanning mutagenesis revealed that Ser77 and Asp215 were critical for both esterase and bioactivity, consistent with prior work implicating a Ser residue [Strickland, J. H., et al. (1995) Plant Physiol. 109, 667-674] and a Ser-Asp dyad [Hirschberg, H. J. H. B., et al. (2001) Eur. J. Biochem. 268, 5037-5044] in patatin's catalytic activity. The crystal structure aids the understanding of other structure-function relationships in patatin. Patatin does not display interfacial activation, a hallmark feature of lipases, and this is likely due to the fact that it lacks a flexible lid that can shield the active site.
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(as it appears on PubMed at http://www.pubmed.gov), where 12779324 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12779324}}
==About this Structure==
==About this Structure==
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[[Category: Alpha/beta class fold with approximately three layer]]
[[Category: Alpha/beta class fold with approximately three layer]]
[[Category: Alpha/beta/alpha in content. possesses a central six-stranded beta sheet with alpha-helices front & back]]
[[Category: Alpha/beta/alpha in content. possesses a central six-stranded beta sheet with alpha-helices front & back]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:19:27 2008''

Revision as of 06:19, 29 July 2008

Image:1oxw.png

Template:STRUCTURE 1oxw

The Crystal Structure of SeMet Patatin

Template:ABSTRACT PUBMED 12779324

About this Structure

1OXW is a Single protein structure of sequence from Solanum cardiophyllum. Full crystallographic information is available from OCA.

Reference

The crystal structure, mutagenesis, and activity studies reveal that patatin is a lipid acyl hydrolase with a Ser-Asp catalytic dyad., Rydel TJ, Williams JM, Krieger E, Moshiri F, Stallings WC, Brown SM, Pershing JC, Purcell JP, Alibhai MF, Biochemistry. 2003 Jun 10;42(22):6696-708. PMID:12779324

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