2lyo

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{{STRUCTURE_2lyo| PDB=2lyo | SCENE= }}
{{STRUCTURE_2lyo| PDB=2lyo | SCENE= }}
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'''CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN 90% ACETONITRILE-WATER'''
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===CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN 90% ACETONITRILE-WATER===
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==Overview==
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Tetragonal crystals of hen egg white lysozyme were cross-linked and subjected to X-ray diffraction study in acetonitrile-water media with different acetonitrile concentrations. Crystals in neat acetonitrile did not scatter X-ray well. Structures of crystals in neat water, in 90% and 95% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% and 95% acetonitrile, only one protein-bond acetonitrile molecule is found in the active site cleft, and its location and binding-protein mode is similar to the C subunit of polysaccharide. The alteration in conformation and hydrogen-bond pattern involving water as solvent causes the reduction of the protein's flexibility in organic media. The back-soaked crystal regained its ordinary three-dimensional structure in water.
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{{ABSTRACT_PUBMED_9659395}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:31:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:19:41 2008''

Revision as of 06:19, 29 July 2008

Image:2lyo.png

Template:STRUCTURE 2lyo

CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN 90% ACETONITRILE-WATER

Template:ABSTRACT PUBMED 9659395

About this Structure

2LYO is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture., Wang Z, Zhu G, Huang Q, Qian M, Shao M, Jia Y, Tang Y, Biochim Biophys Acta. 1998 May 19;1384(2):335-44. PMID:9659395

Page seeded by OCA on Tue Jul 29 09:19:41 2008

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