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1snm

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{{STRUCTURE_1snm| PDB=1snm | SCENE= }}
{{STRUCTURE_1snm| PDB=1snm | SCENE= }}
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'''ACTIVE SITE MUTANT GLU-43 (RIGHT ARROW) ASP IN STAPHYLOCOCCAL NUCLEASE DISPLAYS NONLOCAL STRUCTURAL CHANGES'''
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===ACTIVE SITE MUTANT GLU-43 (RIGHT ARROW) ASP IN STAPHYLOCOCCAL NUCLEASE DISPLAYS NONLOCAL STRUCTURAL CHANGES===
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==Overview==
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The crystal structure of the Glu-43----Asp mutant of staphylococcal nuclease complexed with Ca2+ and the inhibitor thymidine 3',5'-bisphosphate (pdTp) has been determined and refined by restrained least-squares methods to a conventional crystallographic R value of 0.174 at a resolution of 1.74 A. Throughout most of the structure, the conformation of the backbone atoms of the mutant is similar to that of the wild-type protein; however, the seemingly conservative mutation Glu----Asp has significantly perturbed the structure of a loop adjacent to the active site, as well as giving rise to looser binding of the essential calcium ion and to a less extensive network of bound water molecules in the active site. Crystal contacts that extend into the active site have also been altered by this amino acid substitution. The changes caused by this mutation are considerably more drastic than would have been predicted and should serve as caveats to those who would draw conclusions about structure-function relationships on the basis of site-directed mutagenesis experiments in the absence of structural data.
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(as it appears on PubMed at http://www.pubmed.gov), where 2397218 is the PubMed ID number.
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{{ABSTRACT_PUBMED_2397218}}
==About this Structure==
==About this Structure==
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[[Category: Lattman, E E.]]
[[Category: Lattman, E E.]]
[[Category: Loll, P J.]]
[[Category: Loll, P J.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:55:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:20:15 2008''

Revision as of 06:20, 29 July 2008

Image:1snm.png

Template:STRUCTURE 1snm

ACTIVE SITE MUTANT GLU-43 (RIGHT ARROW) ASP IN STAPHYLOCOCCAL NUCLEASE DISPLAYS NONLOCAL STRUCTURAL CHANGES

Template:ABSTRACT PUBMED 2397218

About this Structure

1SNM is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

Active site mutant Glu-43----Asp in staphylococcal nuclease displays nonlocal structural changes., Loll PJ, Lattman EE, Biochemistry. 1990 Jul 24;29(29):6866-73. PMID:2397218

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