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2pjp

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[[Image:2pjp.gif|left|200px]]
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{{STRUCTURE_2pjp| PDB=2pjp | SCENE= }}
{{STRUCTURE_2pjp| PDB=2pjp | SCENE= }}
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'''Structure of the mRNA-binding domain of elongation factor SelB from E.coli in complex with SECIS RNA'''
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===Structure of the mRNA-binding domain of elongation factor SelB from E.coli in complex with SECIS RNA===
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==Overview==
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Elongation factor SelB is responsible for co-translational incorporation of selenocysteine (Sec) into proteins. The UGA stop codon is recoded as a Sec codon in the presence of a downstream mRNA hairpin. In prokaryotes, in addition to the EF-Tu-like N-terminal domains, a C-terminal extension containing four tandem winged-helix motifs (WH1-4) recognizes the mRNA hairpin. The 2.3-A resolution crystal structure of the Escherichia coli WH3/4 domains bound to mRNA with mutagenesis data reveal that the two WH motifs use the same structural elements to bind RNA. The structure together with the 2.6-A resolution structure of the WH1-4 domains from Moorella thermoacetica bound to RNA revealed that a salt bridge connecting WH2 to WH3 modules is disrupted upon mRNA binding. The results provide a structural basis for the molecular switch that may allow communication between tRNA and mRNA binding sites and illustrate how RNA acts as an activator of the switch. The structures show that tandem WH motifs not only provide an excellent scaffold for RNA binding but can also have an active role in the function of protein-RNA complexes.
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{{ABSTRACT_PUBMED_17537456}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB., Soler N, Fourmy D, Yoshizawa S, J Mol Biol. 2007 Jul 20;370(4):728-41. Epub 2007 May 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17537456 17537456]
Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB., Soler N, Fourmy D, Yoshizawa S, J Mol Biol. 2007 Jul 20;370(4):728-41. Epub 2007 May 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17537456 17537456]
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Crystallization and preliminary X-ray analysis of the mRNA-binding domain of elongation factor SelB from Escherichia coli in complex with RNA., Soler N, Fourmy D, Yoshizawa S, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt, 5):419-21. Epub 2007 Apr 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17565186 17565186]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Translation/rna complex]]
[[Category: Translation/rna complex]]
[[Category: Winged-helix]]
[[Category: Winged-helix]]
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Revision as of 06:23, 29 July 2008

Image:2pjp.png

Template:STRUCTURE 2pjp

Structure of the mRNA-binding domain of elongation factor SelB from E.coli in complex with SECIS RNA

Template:ABSTRACT PUBMED 17537456

About this Structure

2PJP is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB., Soler N, Fourmy D, Yoshizawa S, J Mol Biol. 2007 Jul 20;370(4):728-41. Epub 2007 May 10. PMID:17537456

Crystallization and preliminary X-ray analysis of the mRNA-binding domain of elongation factor SelB from Escherichia coli in complex with RNA., Soler N, Fourmy D, Yoshizawa S, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt, 5):419-21. Epub 2007 Apr 20. PMID:17565186

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