1x22

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[[Image:1x22.gif|left|200px]]
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[[Image:1x22.png|left|200px]]
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{{STRUCTURE_1x22| PDB=1x22 | SCENE= }}
{{STRUCTURE_1x22| PDB=1x22 | SCENE= }}
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'''Solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura'''
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===Solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura===
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==Overview==
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An antibacterial peptide was isolated from a lepidopteran insect, Spodoptera litura. The molecular mass of this peptide was determined to be 4489.55 by matrix assisted laser desorption/ionization-time of flight mass (MALDI-TOF MS) spectrometry. The peptide consists of 42 amino acids and the sequence has 69-98% identity to those of moricin-related peptides, antibacterial peptides from lepidopetran insects. Thus, the peptide was designated S. litura (Sl) moricin. Sl moricin showed a broad antibacterial spectrum against Gram-positive and negative bacteria. Sl moricin gene was inducible by bacterial injection and expressed tissue-specifically in the fat body and hemocytes. Furthermore, the solution structure of Sl moricin was determined by two-dimensional (2D) 1H-nuclear magnetic resonance (NMR) spectroscopy and hybrid distance geometry-simulated annealing calculation. The tertiary structure revealed a long alpha-helix containing eight turns along nearly the full length of the peptide like that of moricin, confirming that Sl moricin is a new moricin-like antibacterial peptide. These results suggest that moricin is present not only in B. mori but also in other lepidopteran insects forming a gene family.
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(as it appears on PubMed at http://www.pubmed.gov), where 16115804 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16115804}}
==About this Structure==
==About this Structure==
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1X22 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X22 OCA].
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1X22 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X22 OCA].
==Reference==
==Reference==
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[[Category: Purification]]
[[Category: Purification]]
[[Category: Solution structure]]
[[Category: Solution structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:25:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:31:33 2008''

Revision as of 06:31, 29 July 2008

Image:1x22.png

Template:STRUCTURE 1x22

Solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura

Template:ABSTRACT PUBMED 16115804

About this Structure

1X22 is a Single protein structure. Full experimental information is available from OCA.

Reference

Isolation, gene expression and solution structure of a novel moricin analogue, antibacterial peptide from a lepidopteran insect, Spodoptera litura., Oizumi Y, Hemmi H, Minami M, Asaoka A, Yamakawa M, Biochim Biophys Acta. 2005 Aug 31;1752(1):83-92. PMID:16115804

Page seeded by OCA on Tue Jul 29 09:31:33 2008

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