2j6a

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{{STRUCTURE_2j6a| PDB=2j6a | SCENE= }}
{{STRUCTURE_2j6a| PDB=2j6a | SCENE= }}
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'''CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.'''
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===CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.===
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==Overview==
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Protein release factor eRF1 in Saccharomyces cerevisiae, in complex with eRF3 and GTP, is methylated on a functionally crucial Gln residue by the S-adenosylmethionine-dependent methyltransferase Ydr140w. Here we show that eRF1 methylation, in addition to these previously characterized components, requires a 15-kDa zinc-binding protein, Ynr046w. Co-expression in Escherichia coli of Ynr046w and Ydr140w allows the latter to be recovered in soluble form rather than as inclusion bodies, and the two proteins co-purify on nickel-nitrilotriacetic acid chromatography when Ydr140w alone carries a His tag. The crystal structure of Ynr046w has been determined to 1.7 A resolution. It comprises a zinc-binding domain built from both the N- and C-terminal sequences and an inserted domain, absent from bacterial and archaeal orthologs of the protein, composed of three alpha-helices. The active methyltransferase is the heterodimer Ydr140w.Ynr046w, but when alone, both in solution and in crystals, Ynr046w appears to be a homodimer. The Ynr046w eRF1 methyltransferase subunit is shared by the tRNA methyltransferase Trm11p and probably by two other enzymes containing a Rossman fold.
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{{ABSTRACT_PUBMED_17008308}}
==About this Structure==
==About this Structure==
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[[Category: Transferase]]
[[Category: Transferase]]
[[Category: Translation termination]]
[[Category: Translation termination]]
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Revision as of 06:34, 29 July 2008

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Image:2j6a.png

Template:STRUCTURE 2j6a

CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.

Template:ABSTRACT PUBMED 17008308

About this Structure

2J6A is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast., Heurgue-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH, J Biol Chem. 2006 Nov 24;281(47):36140-8. Epub 2006 Sep 28. PMID:17008308

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