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2ari

From Proteopedia

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[[Image:2ari.gif|left|200px]]
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{{STRUCTURE_2ari| PDB=2ari | SCENE= }}
{{STRUCTURE_2ari| PDB=2ari | SCENE= }}
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'''Solution structure of micelle-bound fusion domain of HIV-1 gp41'''
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===Solution structure of micelle-bound fusion domain of HIV-1 gp41===
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==Overview==
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The N-terminal fusion domain of the HIV-1 gp41 envelope glycoprotein is responsible for initiating the fusion of viral and cellular membranes, leading to the subsequent infection of the host cell by HIV-1. We have investigated the backbone structure and dynamics of the 30 N-terminal residues of HIV-1 gp41 in membrane-mimicking environments using NMR spectroscopy and (15)N- and (15)N,(13)C,(2)H-labeled peptides. Similar (15)N-(1)H HSQC spectra were obtained in a variety of detergents, including SDS, DPC, mixed DPC/SDS, and LPPG micelles, indicating that the peptide structure is not strongly influenced by the type of detergent used. Detailed characterization was carried out in SDS micelles, where the long-term sample stability was found to be optimal. In addition to J-coupling and NOE restraints, a nearly complete set of backbone residual dipolar coupling restraints was recorded for the fusion domain-micelle complex aligned with respect to the magnetic field using a stretched polyacrylamide gel. Backbone amide (15)N spin relaxation and amide hydrogen exchange rates with the solvent were also measured. The ensemble of NMR structures reveals an uninterrupted alpha-helix for the least mobile residues (S(2) &gt; 0.65), Ile-4 to Met-19, with transient helical character extending up to Ala-22. A 12-residue (Ile-4 to Ala-15) segment is fully shielded from solvent, with Gly-3 and Gly-16 found at micelle-solvent interfaces. Residues external to the micelle exhibit enhanced picosecond to nanosecond time scale dynamics relative to the residues buried in the micelle, and their mobility increases with the distance from the micelle.
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{{ABSTRACT_PUBMED_16331977}}
==About this Structure==
==About this Structure==
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2ARI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ARI OCA].
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2ARI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ARI OCA].
==Reference==
==Reference==
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[[Category: Protein]]
[[Category: Protein]]
[[Category: Virus]]
[[Category: Virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:23:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:36:36 2008''

Revision as of 06:36, 29 July 2008

Image:2ari.png

Template:STRUCTURE 2ari

Solution structure of micelle-bound fusion domain of HIV-1 gp41

Template:ABSTRACT PUBMED 16331977

About this Structure

2ARI is a Single protein structure of sequence from Human immunodeficiency virus 1. Full experimental information is available from OCA.

Reference

Structure and dynamics of micelle-associated human immunodeficiency virus gp41 fusion domain., Jaroniec CP, Kaufman JD, Stahl SJ, Viard M, Blumenthal R, Wingfield PT, Bax A, Biochemistry. 2005 Dec 13;44(49):16167-80. PMID:16331977

Page seeded by OCA on Tue Jul 29 09:36:36 2008

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