1nui

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[[Image:1nui.gif|left|200px]]
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{{STRUCTURE_1nui| PDB=1nui | SCENE= }}
{{STRUCTURE_1nui| PDB=1nui | SCENE= }}
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'''Crystal Structure of the primase fragment of Bacteriophage T7 primase-helicase protein'''
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===Crystal Structure of the primase fragment of Bacteriophage T7 primase-helicase protein===
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==Overview==
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DNA primases are template-dependent RNA polymerases that synthesize oligoribonucleotide primers that can be extended by DNA polymerase. The bacterial primases consist of zinc binding and RNA polymerase domains that polymerize ribonucleotides at templating sequences of single-stranded DNA. We report a crystal structure of bacteriophage T7 primase that reveals its two domains and the presence of two Mg(2+) ions bound to the active site. NMR and biochemical data show that the two domains remain separated until the primase binds to DNA and nucleotide. The zinc binding domain alone can stimulate primer extension by T7 DNA polymerase. These findings suggest that the zinc binding domain couples primer synthesis with primer utilization by securing the DNA template in the primase active site and then delivering the primed DNA template to DNA polymerase. The modular architecture of the primase and a similar mechanism of priming DNA synthesis are likely to apply broadly to prokaryotic primases.
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(as it appears on PubMed at http://www.pubmed.gov), where 12769857 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12769857}}
==About this Structure==
==About this Structure==
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[[Category: Transferase]]
[[Category: Transferase]]
[[Category: Zinc-biding domain]]
[[Category: Zinc-biding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:59:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:37:26 2008''

Revision as of 06:37, 29 July 2008

Image:1nui.png

Template:STRUCTURE 1nui

Crystal Structure of the primase fragment of Bacteriophage T7 primase-helicase protein

Template:ABSTRACT PUBMED 12769857

About this Structure

1NUI is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.

Reference

Modular architecture of the bacteriophage T7 primase couples RNA primer synthesis to DNA synthesis., Kato M, Ito T, Wagner G, Richardson CC, Ellenberger T, Mol Cell. 2003 May;11(5):1349-60. PMID:12769857

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