2di2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2di2.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2di2.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2di2| PDB=2di2 | SCENE= }}
{{STRUCTURE_2di2| PDB=2di2 | SCENE= }}
-
'''NMR structure of the HIV-2 nucleocapsid protein'''
+
===NMR structure of the HIV-2 nucleocapsid protein===
-
==Overview==
+
<!--
-
NCp8 of HIV-2 contains two CCHC-type zinc fingers connected by a linker, and is involved in many critical steps of the virus life cycle. It was previously shown that the first zinc finger flanked by the linker is the minimal active domain for specific binding to viral RNA. In our previous study, we determined the three-dimensional structure of NCp8-f1, including the minimal active domain, and found that a hydrogen bond between Asn(11) N(delta)H and Arg(27) O stabilized the conformation of the linker in the vicinity of the zinc finger [Kodera et al. (1998) Biochemistry 37, 17704-17713]. In this study, RNA binding activities of NCp8-f1 and three types of its mutant peptides were analysed by native PAGE assay. The activity and three-dimensional structure of NCp8-f1/N11A, in which alanine is substituted for Asn(11) thereby affecting the conformation of the linker, was analyzed and compared with those of NCp8-f1. We demonstrated that the existence of Arg(4) and/or Lys(5) and Arg(26) and/or Arg(27) were necessary for binding RNA. Furthermore, the linker's flexible orientation, which is controlled by the hydrogen bond between Asn(11) N(delta)H and Arg(27) O, appears to be a structural basis for NCp8 existing as a multi-functional protein.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17202191}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17202191 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17202191}}
==About this Structure==
==About this Structure==
-
2DI2 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DI2 OCA].
+
2DI2 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DI2 OCA].
==Reference==
==Reference==
Line 35: Line 39:
[[Category: Rna recognition]]
[[Category: Rna recognition]]
[[Category: Zinc finger]]
[[Category: Zinc finger]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:27:48 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:44:43 2008''

Revision as of 06:44, 29 July 2008

Image:2di2.png

Template:STRUCTURE 2di2

NMR structure of the HIV-2 nucleocapsid protein

Template:ABSTRACT PUBMED 17202191

About this Structure

2DI2 is a Single protein structure. Full experimental information is available from OCA.

Reference

RNA recognition mechanism of the minimal active domain of the human immunodeficiency virus type-2 nucleocapsid protein., Matsui T, Kodera Y, Endoh H, Miyauchi E, Komatsu H, Sato K, Tanaka T, Kohno T, Maeda T, J Biochem. 2007 Feb;141(2):269-77. Epub 2007 Jan 3. PMID:17202191

Page seeded by OCA on Tue Jul 29 09:44:43 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2di2"
Personal tools