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2vad

From Proteopedia

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{{STRUCTURE_2vad| PDB=2vad | SCENE= }}
{{STRUCTURE_2vad| PDB=2vad | SCENE= }}
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'''MONOMERIC RED FLUORESCENT PROTEIN, DSRED.M1'''
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===MONOMERIC RED FLUORESCENT PROTEIN, DSRED.M1===
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==Overview==
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The red fluorescent protein DsRed has been extensively engineered for use as an in vivo research tool. In fast maturing DsRed variants, the chromophore maturation half-time is approximately 40 min, compared to approximately 12 h for wild-type DsRed. Further, DsRed has been converted from a tetramer into a monomer, a task that entailed mutating approximately 20% of the amino acids. These engineered variants of DsRed have proven extremely valuable for biomedical research, but the structural basis for the improved characteristics has not been thoroughly investigated. Here we present a 1.7 A crystal structure of the fast maturing tetrameric variant DsRed.T4. We also present a biochemical characterization and 1.6 A crystal structure of the monomeric variant DsRed.M1, also known as DsRed-Monomer. Analysis of the crystal structures suggests that rearrangements of Ser69 and Glu215 contribute to fast maturation, and that positioning of the Lys70 side chain modulates fluorescence quantum yield. Despite the 45 mutations in DsRed.M1 relative to wild-type DsRed, there is a root-mean-square deviation of only 0.3 A between the two structures. We propose that novel intramolecular interactions in DsRed.M1 partially compensate for the loss of intermolecular interactions found in the tetramer.
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(as it appears on PubMed at http://www.pubmed.gov), where 17962222 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17962222}}
==About this Structure==
==About this Structure==
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[[Category: Monomeric]]
[[Category: Monomeric]]
[[Category: Photoprotein]]
[[Category: Photoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:29:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:49:11 2008''

Revision as of 06:49, 29 July 2008

Image:2vad.png

Template:STRUCTURE 2vad

MONOMERIC RED FLUORESCENT PROTEIN, DSRED.M1

Template:ABSTRACT PUBMED 17962222

About this Structure

2VAD is a Single protein structure of sequence from Discosoma sp.. Full crystallographic information is available from OCA.

Reference

Structural rearrangements near the chromophore influence the maturation speed and brightness of DsRed variants., Strongin DE, Bevis B, Khuong N, Downing ME, Strack RL, Sundaram K, Glick BS, Keenan RJ, Protein Eng Des Sel. 2007 Nov;20(11):525-34. Epub 2007 Oct 25. PMID:17962222

Page seeded by OCA on Tue Jul 29 09:49:11 2008

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