1oe1

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{{STRUCTURE_1oe1| PDB=1oe1 | SCENE= }}
{{STRUCTURE_1oe1| PDB=1oe1 | SCENE= }}
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'''ATOMIC RESOLUTION STRUCTURE OF THE WILDTYPE NATIVE NITRITE REDUCTASE FROM ALCALIGENES XYLOSOXIDANS'''
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===ATOMIC RESOLUTION STRUCTURE OF THE WILDTYPE NATIVE NITRITE REDUCTASE FROM ALCALIGENES XYLOSOXIDANS===
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==Overview==
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We provide the first atomic resolution (&lt;1.20 A) structure of a copper protein, nitrite reductase, and of a mutant of the catalytically important Asp92 residue (D92E). The atomic resolution where carbon-carbon bonds of the peptide become clearly resolved, remains a key goal of structural analysis. Despite much effort and technological progress, still very few structures are known at such resolution. For example, in the Protein Data Bank (PDB) there are some 200 structures of copper proteins but the highest resolution structure is that of amicyanin, a small (12 kDa) protein, which has been resolved to 1.30 A. Here, we present the structures of wild-type copper nitrite reductase (wtNiR) from Alcaligenes xylosoxidans (36.5 kDa monomer), the "half-apo" recombinant native protein and the D92E mutant at 1.04, 1.15 and 1.12A resolutions, respectively. These structures provide the basis from which to build a detailed mechanism of this important enzyme.
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{{ABSTRACT_PUBMED_12691751}}
==About this Structure==
==About this Structure==
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[[Category: Denitrification]]
[[Category: Denitrification]]
[[Category: Nitrite reductase]]
[[Category: Nitrite reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:43:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:56:46 2008''

Revision as of 06:56, 29 July 2008

Image:1oe1.png

Template:STRUCTURE 1oe1

ATOMIC RESOLUTION STRUCTURE OF THE WILDTYPE NATIVE NITRITE REDUCTASE FROM ALCALIGENES XYLOSOXIDANS

Template:ABSTRACT PUBMED 12691751

About this Structure

1OE1 is a Single protein structure of sequence from Achromobacter xylosoxidans. Full crystallographic information is available from OCA.

Reference

Atomic resolution structures of native copper nitrite reductase from Alcaligenes xylosoxidans and the active site mutant Asp92Glu., Ellis MJ, Dodd FE, Sawers G, Eady RR, Hasnain SS, J Mol Biol. 2003 Apr 25;328(2):429-38. PMID:12691751

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