2gn5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2gn5.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2gn5.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2gn5| PDB=2gn5 | SCENE= }}
{{STRUCTURE_2gn5| PDB=2gn5 | SCENE= }}
-
'''REFINED STRUCTURE OF THE GENE 5 DNA BINDING PROTEIN FROM BACTERIOPHAGE FD'''
+
===REFINED STRUCTURE OF THE GENE 5 DNA BINDING PROTEIN FROM BACTERIOPHAGE FD===
-
==Overview==
+
<!--
-
The three-dimensional structure of the gene 5 DNA binding protein (G5BP) from bacteriophage fd has been determined from a combination of multiple isomorphous replacement techniques, partial refinements and deleted fragment difference Fourier syntheses. The structure was refined using restrained parameter least-squares and difference Fourier methods to a final residual of R = 0.217 for the 3528 statistically significant reflections present to 2.3 A resolution. In addition to the 682 atoms of the protein, 12 solvent molecules were included. We describe here the dispositions and orientations of the amino acid side-chains and their interactions as visualized in the G5BP structure. The G5BP monomer of 87 peptide units is almost entirely in the beta-conformation, organized as a three-stranded sheet, a two-stranded beta-ribbon and a broad connecting loop. There is no alpha-helix present in the molecule. Two G5BP monomers are tightly interlocked about an intermolecular dyad axis to form a compact dimer unit of about 55 A X 45 A X 36 A. The dimer is characterized by two symmetry-related antiparallel clefts that traverse the monomer surfaces essentially perpendicular to the dyad axis. From the three-stranded antiparallel beta-sheet, formed from the first two-thirds of the sequence, extend three tyrosine residues (26, 34, 41), a lysine (46) and two arginine residues (16, 21) that, as indicated by other physical and chemical experiments, are directly involved in DNA binding. Other residues likely to share binding responsibility are arginine 80 extending from the beta-ribbon and phenylalanine 73 from the tip of this loop, but as provided, however, by the opposite monomer within each G5BP dimer pair. Thus, both symmetry-related DNA binding sites have a composite nature and include contributions from both elements of the dimer. The gene 5 dimer is clearly the active binding species, and the two monomers within the dyad-related pair are so structurally contiguous that one cannot be certain whether the isolated monomer would maintain its observed crystal structure. This linkage is manifested primarily as a skeletal core of hydrophobic residues that extends from the center of each monomer continuously through an intermolecular beta-barrel that joins the pair. Protruding from the major area of density of each monomer is an elongated wing of tenuous structure comprising residues 15 through 32, which is, we believe, intimately involved in DNA binding. This wing appears to be dynamic and mobile, even in the crystal and, therefore, is likely to undergo conformational change in the presence of the ligand.
+
The line below this paragraph, {{ABSTRACT_PUBMED_6684697}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 6684697 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_6684697}}
==About this Structure==
==About this Structure==
Line 24: Line 28:
[[Category: Brayer, G D.]]
[[Category: Brayer, G D.]]
[[Category: McPherson, A.]]
[[Category: McPherson, A.]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:17:51 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:58:06 2008''

Revision as of 06:58, 29 July 2008

Image:2gn5.png

Template:STRUCTURE 2gn5

REFINED STRUCTURE OF THE GENE 5 DNA BINDING PROTEIN FROM BACTERIOPHAGE FD

Template:ABSTRACT PUBMED 6684697

About this Structure

2GN5 is a Single protein structure of sequence from Enterobacteria phage m13. This structure supersedes the now removed PDB entry 1gn5. Full crystallographic information is available from OCA.

Reference

Refined structure of the gene 5 DNA binding protein from bacteriophage fd., Brayer GD, McPherson A, J Mol Biol. 1983 Sep 15;169(2):565-96. PMID:6684697

Page seeded by OCA on Tue Jul 29 09:58:06 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gn5"
Personal tools